Characterisation and Analysis of Chitosan Extract from Artemia franciscana Using SEM, FT-IR and XRD Studies


Published: 2023-06-10

DOI: 10.56557/upjoz/2023/v44i113515

Page: 15-24

J. Joonu *

Department of Zoology, Bishop Heber College, Trichy-17, India.

Saravana Kumar

Department of Zoology, Bishop Heber College, Trichy-17, India.

*Author to whom correspondence should be addressed.


Introduction: Artemia franciscana is one of the best animal models which is used in various fields. After the hatching of Artemia the shell wastes are used for Chitosan extraction. The extraction process consumes less time when compare with other shells because of its shell size.

Materials and Methods: The Chitosan extract was prepared using Artemia shells. The Physico-chemical properties were analysed and characterization of Chitosan extract was done using SEM, XRD and FT-IR studies.The physicochemical properties of the extracted chitosan indicate that it has optimum values desirable for wound healing applications. Chitosan has high water and fat binding capacity that can help to maintain a moist environment on the wound surface and control inflammation, respectively. The degree of deacetylation (DD) of chitosan is critical in determining its biological activities. The DD value obtained in this study was 84.77%, indicating that the chitosan sample had a high degree of deacetylation. Outcomes: The morphology of the chitosan membrane is an important factor that influences its properties and potential applications, including wound healing.

Results: The SEM images obtained in this study revealed that pure chitosan exhibited a nonporous, smooth membranous phase with dome-shaped orifices, microfibrils, and crystallites. The XRD analysis results suggest that the chitosan used in this study is highly crystalline, with both crystalline and amorphous regions enhancing its bioactivity and biocompatibility, making it a promising material for wide range of application in medicine. The crystalline and amorphous structure of Chitosan is analysed by the XRD, SEM and FT-IR reveals its wide range of applications in medicine.

Keywords: Artemia franciscana, chitosan, extraction, XRD analysis

How to Cite

Joonu , J., & Kumar , S. (2023). Characterisation and Analysis of Chitosan Extract from Artemia franciscana Using SEM, FT-IR and XRD Studies. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(11), 15–24.


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Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH, Boyd MR. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res; 1988.

Carmichael J, Mitchell JB, DeGraff WG, Gamson J, Gazdar AF, Johnson BE, Glatstein E, Minna JD. Chemosensitivity testing of human lung cancer cell lines using the MTT assay. Br. J. Cancer. 1988;57:540-547.

Rudall KM, Kenchington W. The chitin system. Biol Rev. 1973;40:597– 636.

Abdou ES, Nagy KSA, Elsabee MZ. Extraction and characterization of chitin and chitosan from local sources. Bioresource Technol. 2008;99:1359-1367.

Chandumpai A, Singhpibulporn N, Faroongsarng D, Sornprasit P. Preparation and physicochemical characterization of chitin and chitosan from the pens of the squid species, Loligo lessoniana and Loligo formosana. Carbohyd Polym. 2004;58:467–474.

Synowiecki J, Al-Khateeb NA. Production, properties, and some new applications of chitin and its derivatives. Crit. Rev. Food. Sci. Nutr; 2003.

Asadpour Y. Biotechnological approach for production of chitin and chitosan from Artemia urmiana cyst shell. Ph.D Dissertation, Tarbiat Modarres University: Tehran, Iran; 2003.

Kumari S, Rath P, Hari AS, Tiwari TN. Extraction and characterization of chitin and chitosan from fishery waste by chemical method, Environ. Technol. Innov. 2015;3:77–85. DOI:10.1016/j.eti.2015.01.002.

Bajaj M, Winter J, Gallert C. Effect of deproteination and deacetylation conditions on viscosity of chitin and chitosan extracted from Crangon crangon shrimp waste. Biochem. Eng. J. 2011;56: 51–62. DOI:10.1016/j.bej.2011.05.006.

Kumari S, Rath PK. Extraction and characterization of chitin and chitosan from (Labeo rohit) Fish Scales. Procedia Mater. Sci. 2014;6:482– 489. DOI:10.1016/j.mspro.2014.07.062

Castro SPM, Paulín EGL. Is chitosan a new panacea ? Areas of Application. 2012;3–46.

Kannan M, Nesakumari M, Rajarathinam K. Production and characterization of mushroom chitosan under solid-state fermentation conditions department of advanced zoology and bio-technology. Sri Paramakalyani College, Adv. Biol. Res. (Rennes). 2010;4:10–13.

Blackwell J. Chitin. In: Walton AG, Blackwell J, editors. Biopolymers. New York: Academic Press. 1973; 474–89.

Rudall KM. Chitin and its association with other molecules. J Polym Sci Part C 1969;28:83–102.

Atkins EDT. Conformation in polysaccharides and complex carbohydrates. J Biosci 1985;8:375–87.

Olivera BM, Hillyard DR, Marsh M, Yoshikami D. Combinatorial peptide libraries in drug design: lesson from venomous cone snails. TIBTECH. 1995;13:422–6.

Dutta PK, Dutta J, Tripathi VS. Chitin and chitosan: Chemistry, properties and applications. J. Sci. Ind. Res. 2004;63:20–31.

Ruben RL, Neubauer RH. Semiautomated calorimetric assay for in vitro screening of anticancer compounds. Cancer Treat; 1987.

Agh N, Sorgeloos P. INCO-DEV project on Artemia biodiversity international workshop. Urmia, Iran; 2004.

Tajik H, Moradi M, Razavi Rohani SM, Erfani AM, Jalali FSS. Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physicochemical and functional properties of the product. Molecules. 2008;13(6):1263-1274.

Wang JC, Kinsella JE. Functional properties of novel proteins: Alfalfa leaf protein. Journal of Food Science. 1976;41:286–292.

The United States Pharmacopeial Convention. American pharmacopoeia. The United States Pharmacopeial Convention; New York, NY, USA. 2012; 36:1754–1756.