Phyto Fabrication of Silver Nanocomposites from Ageratum conyzoides as Potent Mosquitocidal and Antidengue Activity of Aedes aegypti


Published: 2023-06-23

DOI: 10.56557/upjoz/2023/v44i123535

Page: 47-60

Christina Mary Samidoss *

Department of Zoology, Michel Job College of Arts and Science for Women, Sulur, Coimbatore, Tamilnadu, India.

Maryjoy Samidoss

PSG Arts and Science, Coimbatore, Tamilnadu, India.

Juliet Gnana Sundari

JCT College of Engineering and Technology, Pichanur, Tamilnadu, India.

*Author to whom correspondence should be addressed.


Mosquitoes pose an enormous threat to millions of people worldwide and transmit important diseases, including malaria, dengue, yellow fever, filariasis, Japanese encephalitis and the Zika virus. Currently, a growing number of phyto-synthesized silver nanoparticles (AgNPs) have recently been proposed as effective mosquito larvicides and are gaining traction over synthetic chemical pesticides due to their less deleterious effects on non-target species and novelty in mechanisms. action. The current study was conducted to evaluate the larvicidal and pupicidal activity of AgNP synthesized from Ageratum conyzoides against dengue vector Aedes aegypti as well as in vitro antiviral assay. The biosynthesized AgNPs were characterized using a UV-Vis spectrometer, powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The mosquito larvae were tested with biosynthesized AgNPs and the LC50 values recorded were I-stage (20.451), II-stage (23.307), III-stage (27.397), IV-stage (33.351), and pupa (39.668), respectively. To screen the anti-dengue properties of A. conyzoides-synthesized AgNP, an in vitro antiviral assay was performed. The present research reported moderate cytotoxicity rates in Vero cells exposed to A. conyzoides-synthesized AgNP at various concentrations. From this we observed that no adverse morphological differences were found in the treated cells that were comparable to the control Vero cells. Overall, our results demonstrated that AgNPs synthesized by A. conyzoides can be used to design newer and safer dengue control agents.

Keywords: Aedes aegypti, silver nanopaticles, green synthesis, larvicidal;pupicidal, invitro analysis, eco-friendly

How to Cite

Samidoss , C. M., Samidoss , M., & Sundari , J. G. (2023). Phyto Fabrication of Silver Nanocomposites from Ageratum conyzoides as Potent Mosquitocidal and Antidengue Activity of Aedes aegypti . UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(12), 47–60.


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Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes et al. The global distribution and burden of dengue. Nature. 2013;496(7446):504-507. DOI:

WHO. Dengue and severe dengue. Fact sheet N117; 2015. Updated May 2015

Sujitha V, Murugan K, Dinesh D, Pandiyan A, Aruliah R, Hwang JS, et al. Green-synthesized CdS nano-pesticides: toxicity on young instars of malaria vectors and impact on enzymatic activities of the non-target mud crab Scylla serrata. Aquatic Toxicology. 2017;188:100-108.DOI:

Amerasan D, Nataraj T, Murugan K, Panneerselvam C, Madhiyazhagan P, Nicoletti M et al. Myco-synthesis of silver nanoparticles using Metarhizium anisopliae against the rural malaria vector Anopheles culicifacies Giles (Diptera: Culicidae). Journal of Pest Science. 2016;89(1):249-256.DOI:

Kumar S, Lather V, Pandita D. Green synthesis of therapeutic nanoparticles: an expanding horizon. Nanomedicine. 2015;10(15):2451-2471. DOI:

Kamboj A, Saluja AK. Ageratum conyzoides L.: A review on its phytochemical and pharmacological profile. International Journal of Green Pharmacy (IJGP). 2008.2(2). DOI:

Sridharan S, Meenaa V, Kavitha V, Nayagam AAJ. GC-MS study and phytochemical profiling of Mimosa pudica Linn. Journal of Pharmacy Research. 2011;4(3):741-2.

Dinesh D, Murugan K, Madhiyazhagan P, Panneerselvam C, Mahesh Kumar P, Nicoletti M, et al. Mosquitocidal and antibacterial activity of green-synthesized silver nanoparticles from Aloe vera extracts: towards an effective tool against the malaria vector Anopheles stephensi?. Parasitology Research. 2015;114:1519-1529.

Kovendan K, Chandramohan B, Dinesh D, Abirami D, Vijayan P, Govindarajan M, et al. Green-synthesized silver nanoparticles using Psychotria nilgiriensis: toxicity against the dengue vector Aedes aegypti (Diptera: Culicidae) and impact on the predatory efficiency of the non-target organism Poecilia sphenops (Cyprinodontiformes: Poeciliidae). Journal of Asia-Pacific Entomology. 2016;19(4):1001-1007. DOI:

Han C, Shao H, Zhou S, Mei Y, Cheng Z, et al. Chemical composition and phytotoxicity of essential oil from invasive plant Ambrosia artemisiifolia L. Ecotoxicology and Environmental Safety. 2021;211:111879.


Kalaimurugan D, Vivekanandhan P, Sivasankar P, Durairaj K, Senthilkumar P, Shivakumar MS, et al. Larvicidal activity of silver nanoparticles synthesized by Pseudomonas fluorescens YPS3 isolated from the Eastern Ghats of India. Journal of Cluster Science. 2019;30(1):225-233. DOI:

Pirtarighat S, Ghannadnia M, Baghshahi S. Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry. 2019;9(1):1-9. DOI:

Vinoth S, Shankar S.G, Gurusaravanan P, Janani B, Devi JK. Anti-larvicidal activity of silver nanoparticles synthesized from Sargassum polycystum against mosquito vectors. Journal of Cluster Science. 2019;30(1):171-180.


Lagashetty A, Patil MK, Ganiger SK. Green synthesis characterization and thermal study of silver nanoparticles by Achras sapota Psidium guajava and Azadirachta indica plant extracts. Plasmonics. 2019;14(5):1219-1226.


Oves M, Aslam M, Rauf M.A, Qayyum S, Qari HA, et al. Antimicrobial and anticancer activities of silver nanoparticles synthesized from the root hair extract of Phoenix dactylifera. Materials Science and Engineering: C. 2018;89:429-443. DOI:

Estévez M, Luna C. Dietary protein oxidation: A silent threat to human health?. Critical Reviews in Food Science and Nutrition. 2017;57(17):3781-3793. DOI:

Shanmuganathan R, MubarakAli D, Prabakar D, Muthukumar H, Thajuddin N, Kumar SS, et al. An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach. Environmental Science and Pollution Research. 2018;(11):10362-10370. DOI:

Ga’al H, Yang G, Fouad H, Guo M, Mo J. Mannosylerythritol lipids mediated biosynthesis of silver nanoparticles: an eco-friendly and operative approach against chikungunya vector Aedes albopictus. Journal of Cluster Science. 2021;32(1):17-25. DOI:

Nalini M, Lena M, Sumathi P, Sundaravadivelan C. Effect of phyto-synthesized silver nanoparticles on developmental stages of malaria vector Anopheles stephensi and dengue vector Aedes aegypti. Egyptian Journal of Basic and Applied Sciences. 2017;4(3):212-218. DOI:

Yogarajalakshmi P, Poonguzhali TV, Ganesan R, Karthi S, Senthil-Nathan S, Krutmuang P, et al.. Toxicological screening of marine red algae Champia parvula (C. Agardh) against the dengue mosquito vector Aedes aegypti (Linn.) and its non-toxicity against three beneficial aquatic predators. Aquatic Toxicology. 2020;222:105474.


Paulpandi M, Kavithaa K, Sumathi S, Padma PR. Increased anticancer efficacy by the combined administration of quercetin in multidrug resistant breast cancer cells. Annals of Oncology. 2013; 24:iii19.


Sujitha V, Murugan K, Paulpandi M, Panneerselvam C, Suresh U, Roni M, et al. Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti. Parasitology Research. 2015;114(9:3315-3325. DOI:

Murugan K, Benelli G, Panneerselvam C, Subramaniam J, Jeyalalitha T, Dinesh D, et al. Cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod Mesocyclops aspericornis against malaria and dengue mosquitoes. Experimental Parasitology. 2015;153:129-138.


Murugan K, Aruna P, Panneerselvam C, Madhiyazhagan P, Paulpandi M, Subramaniam J, et al. Fighting arboviral diseases: low toxicity on mammalian cells dengue growth inhibition (in vitro) and mosquitocidal activity of Centroceras clavulatum-synthesized silver nanoparticles. Parasitology Research. 2016;115(2):651-662.


Benelli G. Plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review. Parasitology Research. 2015;114(9):3201-3212. DOI:

Benelli G Plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review. Parasitology Research. 2016;115(1):23-34. DOI: