Changes in Ascorbic Acid in Liver, Muscle and Gills Due to Dimethoate Induced in Freshwater Fish Puntius ticto

R. Y. Bhandare

Department of Zoology, Mahatma Gandhi Vidyamandir’s Arts, Science and Commerce College, Surgana, District Nashik-422211, MS, India.

K. T. Paithane

Department of Zoology, Deogiri College, Aurangabd, India.

P. S. Kharat

Department of Zoology, Nutan Mahavidyalaya, Selu, Dist. Parbhani, MS, India.

T. S. Pathan *

Department of Zoology, Kalikadevi Arts, Commerce and Science College, Shirur (Ka), Dist. Beed, MS, India.

*Author to whom correspondence should be addressed.


In the present investigation, ascorbic acid content was observed in the liver, muscle and gills in the Punctius ticto fishes, exposed to sub lethal concentration of dimethoate. Ascorbic acid is a key antioxidant in the liver, gill and muscles of fish. Intrinsic properties of ascorbic acid beyond acting as an antioxidant are important in its role as a key molecule in the metabolism of fish.

The present research work deals with the evaluation of the ascorbic acid on dimethoate induced in Puntius ticto. The dimethoate pesticide EC 30% was administered directly in the water contained in the aquarium at the dose of 1.3 ppm (1/5), 0.63 ppm (1/10) and 0.43 ppm (/15) respectively for 96 hrs hours after the dose calculation through LC50. Thereafter, ascorbic acid was administered to each pesticide treated group.  All over the experiment the ascorbic acid content gradually increased in the liver, muscle and slightly decreased in the gills of the intoxicated fishes.

Keywords: Puntius ticto, dimethoate, ascorbic acid, liver, muscle, gills

How to Cite

Bhandare , R. Y., Paithane , K. T., Kharat , P. S., & Pathan , T. S. (2023). Changes in Ascorbic Acid in Liver, Muscle and Gills Due to Dimethoate Induced in Freshwater Fish Puntius ticto. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(18), 71–77.


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Shinde BR. Protective Role of Ascorbic Acid on Cypermethrin Induced Alterations In the Protein Levels of the Freshwater Fish, Puntius Sophore. JETIR. 2018; 5(4):981-986.

Olea N, Fernandez MF. Chemicals in environment and human male fertility. Occupational and Environmental Medicine. 2007;64:430-431.

Geter DR, Kan HL, Lowe ER, Rick DL, Charles GD, Gollapudi BB and Materson JL. Investigations of oxidative stress, antioxidant response and protein binding in cholrpyrifosexposedrat neuronal PC12 cells. Toxicology Mechanisms and Methods. 2008;18:17-23.

El Gendy AS, Aly NM, Mahmoud FH, Kenawy A. and El- Sebae AKH. The role of vitamin C as antioxidant in protection against endogenous oxidative DNA damage in human sperm. Proceedings of the National Academy of Sciences of the USA88. 2010;11003-11006.

Manduzia H, Rocher B, Durand F, Galap C. and Leboulenger F. The point about oxidative stress in molluscs. International Survival Journal. 2005;2:91-104.

Ray SNC, Sinha RC. Electrophoretic variation of creatine kinase isozymes as a function of methyl parathion in the fish, Labeo rohita.IOSR Journal of Environmental Science, Technology and Food Technology. 2016;10: 1-6.

DOI:10.9790/2402- 1007xxxxx

Agarwal R, Tripathi AK, and Chakrabarty AK. Effect of ascorbic acid on stimulatory status of activated mouse peritoneal phagocytosis. Indian Journal of Experimental Biology. 2003;41:290-295.

Holloway DE, Paterson FJ. Acsorbic acid in drug metabolism in drugs and nutrients: The initiative effects. Marcel Dekker, New York & Basel 1984;3:225-235.

Acharya UR, Mishra M, Patro J, Panda MK. Effect of vitamin C and E on spermatogenesis in mice exposed to cadmium. Reproductive Toxicology. 2008;5:84-88.

Ray SNC and Sinha RC. Role of Ascorbic acid in the brain and gills of fish, Labeo rohita and Cirrhinus mrigala following the sub- acute exposure of methyl parathion. International Global Journal for Research Analysis. 2017;6(9):2277-8160.

Sinha RC. Effect of starvation on the concentration of ascorbic acid in the pedipalp muscle of thescorpion, Palamnaeusbengalensis.Experientia.1966;22:1-2.

Ray SNC and Sinha RC. Effect of methyl parathion on the carbohydrate metabolism of the fish, Cirrhinus mrigala. Journal of Materials Science and Engineering (U.S.A.). 2014;B4:366-371.

Ray SNC, Sinha RC. Role of ascorbic acid in the brain and gills of sh, Labeo rohita and Cirrhinus mrigala following the sub-acute exposure of methyl parathion. 2017;6(9). ISSN No 2277 – 8160

Paithane KT, Bhandare RY, Shinde SE, More PR, Pathan TS. Biochemical alterations due to dimethoate in fresh water fish, Channa punctatus. Flora and Fauna. 2021;27(2):316-320.

Rose JH. Methods of biochemical analysis. (Ed. By Glick Intersciences, New York). 1967;5:44.

Katagi T. Bioconcentration, bioaccumulation, and metabolism of pesticides in aquatic organisms. Reviews of Environmental Contamination and Toxicology. Springer, New York, NY. 2010;204:1-132. Available:

Hu L, Luo D, Zhou T, Tao Y, Feng J, Mei S. The association between non-Hodgkin lymphoma and organophosphate pesticides exposure: A meta-analysis. Environmental Pollution. 2017;231(Pt1): 319‐328.


Clasen B, Loro VL, Murussi CR, Tiecher TL, Moraes B, and Zanella R. Bioaccumulation and oxidative stress caused by pesticides in Cyprinus carpio reared in a rice-fish system. Science of the Total Environment. 2018;626:737-743. Available: nv.20 18.01.154.

Sabarwal A, Kumar K, and Singh RP. Haz-ardous effects of chemical pesticides on human health-Cancer and other associated disorders. Environmental Toxicology and Pharmacology. 2018;63: 103-114. Available:https://doi:10.1016/j.etap.2018.08.018.

Mojiri A, Zhou JL, Robinson B, Ohashi A, Ozaki N, Kindaichi T, and Vakili M. Pesticides in aquatic environments and their removal by adsorption methods. Chemosphere. 2020;126646.


Yang C, Lim Wand Song G. Mediation of oxidative stress toxicity induced by pyrethroid pesticides in fish. Comparative Biochemistry and Physiology. Toxicology & Pharmacology. 2020;234:108758.


Sharma G, Singh S. Studies on the effect of intoxicant indofil on the blood morphology of Channa punctatus (Bloch.). Bionotes. 2004;6(1):20.

Sharma G, Singh S. Assay of some blood parameters of the fish, Channa punctatus (Bloch.) after intoxication of indofil. Bionotes. 2006;8(1):21.

Ramesh M, Sankaran M, Veera-Gowtham V, and Poopal R. KHematological, biochemical and enzymological responses in an Indian major carp Labeo rohita induced by sublethal concentration of waterborne selenite exposure. Chemico-biological Interactions. 2014;207:67–73.


Woo SJ, Kim NY, Kim SH, Ahn SJ, Seo JS, Jung SH, Cho MY, and Chung JK. Toxico-logical effects of trichlorfon on hematological and bio-chemical parameters in Cyprinus carpio L. following ther-mal stress. Comparative Biochemistry and Physiology. Toxicology & Pharmacology. 2018;209:18–27.


Bojarski B, Witeska M. Blood biomarkers of herbicide, insecticide, and fungicide toxicity to fish -A re-view. Environmental Science and Pollution Research. 2020;1-15.


Sanjay Kumar Jigyasu and Dilip Kumar Paul. Protective effect of ascorbic acid against fenvalerate induced toxicity in air-breathing fish Clarias batrachus; Journal of Applied and Natural Science. 2020;12(3):380 – 387.

Patel G and Rao MV. Role of ascorbic acid on mercuric chloride toxicity in vital organs of mice. Indian Journal of Environmental Toxicology. 1999;9:53-55.

Gould BS. Collagen Formation and fibrogenesis with special reference to the role of ascorbic acid Gut. Rev. Cytol.1963;15:301-363.

Padhi SM. and Patnaik BK. Changes in ascorbic acid content of kidney following partial hepatectomy in garden lizard Calotes versicolor (Daudin). Ind. J. Exp. Biol. 1978;16:1499.

Wagh SB. and Khillare YK. Effect of malathion on level of ascorbic acid in the freshwater fish, Puntius ticto (Ham.). Marath. Univ. J. of Sci., 1985;86(24-25):85-87.

Bhusari NB. Studies on the effect of pesticide on freshwater fish of Marathwada region. Ph. D. thesis, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, (M.S.) India; 1985.

Davane PM. Impact assessment of pesticides in aquatic animals special reference with fishes. Ph.D. Thesis, Dr. B.A.M.University, Aurangabad (M.S.) India; 1991.

Andersson T, Forlin L, Hardig J and Larsson A. Physiological disturbances in fish living in costal water polluted with bleached kraft mill effluent. Can. J. Fish. Aquat. Sci. 1988;45:1525-1536.

Sayeed I, Parvez S, Pandey S, Bin-Hafeez, B, Haque R and Raisuddin S. Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus. Ecotoxicol Environ Saf. 2003;56:295-301.

Datta M, Kaviraj A. Dietary ascorbic acid supplement to ameliorate chronic toxicity of fenvalerate to Clarias gariepinus. Australian Journal of Ecotoxicology. 2006; 12:129-134.

Paratheswararao V. Some mechanisms underlying thermal acclimation in a freshwater fish, Etroplus maculates (Teleostei). Comp. Biochem. Physiol. 1967;21:619-626.

Somasundaram k, Damodaran VN, Venkitasubramanian TA. Biochemical changes following absorption of dieldrin through skin. Indian J. Expt. Boil. 1978; 16(9):1003-1004.

Ali SM, Ilyas R. and Bhusari NB. Effect of lethal and sublethal concentrations of dimecron on an ascorbic acid content of freshwater, Channa gachua (Ham.) Silver Jubilee Issue, Dr. Babasaheb Ambedkar Marathwada University, 1983;21(15): 69-74.

May JM. Vitamin C transport and its role in the central nervous system. Subcellular Biochemistry. 2012;56:85-103.