Development and Study of Mosquitocidal and Antibacterial Properties of Typhonium trilobatum-Synthesized ZnO NPS and their Impact on the Predation Efficiency of Guppy Poecilia reticulata against the Dengue vector, Aedes aegypti

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Published: 2023-09-22

DOI: 10.56557/upjoz/2023/v44i213664

Page: 16-30


M. Flory Shobana

Department of Biotechnology, RVS College of Arts and Science, Coimbatore, India and Department Biotechnology, Hindusthan College of Arts and Science, Coimbatore, India.

M. P. Ayyappadas *

Department of Biotechnology, RVS College of Arts and Science, Coimbatore, India.

*Author to whom correspondence should be addressed.


Abstract

Dengue fever is an arbovirus spread mostly by Aedes mosquitos. Every year, there is an increase in the incidence of dengue infection and transmission. As a result, the need for an effective measure remains a top concern. The current work aims to assess the effectiveness of Typhonium trilobatum leaf extract synthesized zinc oxide nanoparticles (ZnO NPs) against the Dengue vector, Aedes aegypti. We synthesized zinc nanoparticles by reducing and stabilizing it using T. trilobatum leaf extract. UV-vis spectrophotometry, Fourier transform infrared spectroscopy, X-ray diffraction, energy dispersive X-ray analysis, field emission scanning electron microscopy, transmission electron microscopy and EDAX analysis were used for analyzing nanoparticles. T. trilobatum leaf extract and biosynthesized ZnONPs were extremely efficient against Ae. aegypti young instars, with LC50 values ranging from 36.633 ppm (larva I) to 102.436 ppm (pupa) and 1.555 ppm (larva I) to 6.906 ppm (pupa). The predation effectiveness of Poecillia reticulata guppy fish against A. aegypti I - IV instar larvae were 49.8 and 25.9 %, respectively. Predation was 78.3 and 39.8 % in ZnO-contaminated environments, respectively. The results of T. trilobatum leaf extract synthesized ZnONPs showed considerable larvicidal action with an increase in predatory potential of the guppy fish P. reticulata. To screen the antibacterial activities of T. trilobatum leaf extract and Biosynthesized ZnO NPs against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi, the agar disk diffusion and minimum inhibitory concentration procedures were used. The biosynthesized ZnO NPs showed excellent antibacterial effects against bacterial pathogens. Overall, our study suggests that T. trilobatum leaf extract synthesized ZnO NPs could be used in mosquito control as well as the creation of novel chemotherapeutic medicines with low systemic toxicity.

Keywords: Aedes aegypti, antibacterial activity, mosquitocidal activity, predation efficiency, ZnO Nanoparticles


How to Cite

Shobana , M. F., & Ayyappadas , M. P. (2023). Development and Study of Mosquitocidal and Antibacterial Properties of Typhonium trilobatum-Synthesized ZnO NPS and their Impact on the Predation Efficiency of Guppy Poecilia reticulata against the Dengue vector, Aedes aegypti. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(21), 16–30. https://doi.org/10.56557/upjoz/2023/v44i213664

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References

Morejon B, Pilaquinga F, Domenech F, Ganchala D, Debut A, Neira M. Larvicidal activity of silver nanoparticles synthesized using extracts of Ambrosia arborescens (Asteraceae) to control Aedes aegypti L.(Diptera: Culicidae). Journal of Nanotechnology. 2018;2018.

Ga’al H, Fouad H, Mao G, Tian J, Jianchu M. Larvicidal and pupicidal evaluation of silver nanoparticles synthesized using Aquilaria sinensis and Pogostemon cablin essential oils against dengue and zika viruses vector Aedes albopictus mosquito and its histopathological analysis. Artificial Cells, Nanomedicine, and Biotechnology. 2018;46(6):1171-9.

Govindarajan M, Rajeswary M, Senthilmurugan S, Vijayan P, Alharbi NS, Kadaikunnan S, Khaled JM, Benelli G. Larvicidal activity of the essential oil from Amomum subulatum Roxb.(Zingiberaceae) against Anopheles subpictus, Aedes albopictus and Culex tritaeniorhynchus (Diptera: Culicidae), and non-target impact on four mosquito natural enemies. Physiological and Molecular Plant Pathology. 2018;101:219-24.

Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food and Chemical Toxicology. 2008;46(2):446-75.

Ali K, Ashraf A, Biswas NN. Analgesic, anti-inflammatory and anti-diarrheal activities of ethanolic leaf extract of Typhonium trilobatum L. Schott. Asian Pacific Journal of Tropical Biomedicine. 2012 Sep 1;2(9):722-6.

Tran K. Medicinal Plants in Viet Nam. WHO Regional Office for the Western Pacific Manila and Institute of Material Medica Hanoi, Science and Technology Publishing House, Hanoi. 1990;271.

Chattopadhyay PR, Mukhopadhyaya MC. Comparative studies on the nematicidal properties of Typhonium trilobatum and Melia azedarach. Indian Journal of Nematology. 1989;19(1):5-9.

Haldar KM, Ghosh P, Chandra G. Evaluation of target specific larvicidal activity of the leaf extract of Typhonium trilobatum against Culex quinquefasciatus Say. Asian Pacific Journal of Tropical Biomedicine. 2011;1(2):S199-203.

Kandhasamy M, Arunachalam KD. Efficacy of Typhonium trilobatum (L.) Schott tuber extracts on pathogenic bacteria. Elect J Nat Subs. 2008;3:1-7.

Lima SL, Dill LM. Behavioral decisions made under the risk of predation: A review and prospectus. Canadian Journal of Zoology. 1990;68(4):619-40.

Houston A, Clark C, McNamara J, Mangel M. Dynamic models in behavioural and evolutionary ecology, Nature. 1988; 332(6159):29-34.

Lima SL. Nonlethal effects in the ecology of predator-prey interactions. Bioscience. 1998;48(1):25-34.

Cavalcanti LP, Pontes RJ, Regazzi AC, Paula Júnior FJ, Frutuoso RL, Sousa EP, Dantas Filho FF, Lima JW. Efficacy of fish as predators of Aedes aegypti larvae, under laboratory conditions. Revista de Saude Publica. 2007;41:638-44.

Subramaniam J, Murugan K, Panneerselvam C, Kovendan K, Madhiyazhagan P, Dinesh D, Kumar PM, Chandramohan B, Suresh U, Rajaganesh R, Alsalhi MS. Multipurpose effectiveness of Couroupita guianensis-synthesized gold nanoparticles: High antiplasmodial potential, field efficacy against malaria vectors and synergy with Aplocheilus lineatus predators. Environmental Science and Pollution Research. 2016;23:7543-58.

Morgan DL, Gill HS, Maddern MG, Beatty SJ. Distribution and impacts of introduced freshwater fishes in Western Australia. New Zealand Journal of Marine and Freshwater Research. 2004;38(3):511- 23.

Fernando GK, Jayakody S, Wijenayake WM, Galappaththy GN, Yatawara M, Harishchandra J. A comparison of the larvivorous habits of exotic Poecilia reticulata and native Aplocheilus parvus. BMC ecology. 2018;18(1):1-2.

Phukon HK, Biswas SP. An investigation on larvicidal efficacy of some indigenous fish species of Assam, India. Adv Biores. 2013;4(3):22-5.

Salata OV. Applications of nanoparticles in biology and medicine. Journal of Nanobiotechnology. 2004;2(1):1-6.

Dias AM, Hussain A, Marcos AS, Roque AC. A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharides. Biotechnology Advances. 2011;29(1):142-55.

Rawani A, Ghosh A, Chandra G. Mosquito larvicidal and antimicrobial activity of synthesized nano-crystalline silver particles using leaves and green berry extract of Solanum nigrum L. (Solanaceae: Solanales). Acta Tropica. 2013;128(3):613-22.

Ramimoghadam D, Bin Hussein MZ, Taufiq-Yap YH. Hydrothermal synthesis of zinc oxide nanoparticles using rice as soft biotemplate. Chemistry Central Journal. 2013;7:1-0.

Murugan K, Benelli G, Panneerselvam C, Subramaniam J, Jeyalalitha T, Dinesh D, Nicoletti M, Hwang JS, Suresh U, Madhiyazhagan P. Cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod Mesocyclops aspericornis against malaria and dengue mosquitoes. Experimental Parasitology. 2015;153:129-38.

Suresh U, Murugan K, Benelli G, Nicoletti M, Barnard DR, Panneerselvam C, Kumar PM, Subramaniam J, Dinesh D, Chandramohan B. Tackling the growing threat of dengue: Phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector Aedes aegypti (Diptera: Culicidae). Parasitology Research. 2015; 114:1551-62.

Murugan K, Dinesh D, Paulpandi M, Subramaniam J, Rakesh R, Amuthavalli P, et al. Mangrove helps: Sonneratia alba-synthesized silver nanoparticles magnify guppy fish predation against Aedes aegypti young instars and down-regulate the expression of envelope (E) gene in dengue virus (serotype DEN-2). Journal of Cluster Science. 2017;28:437-61.

Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. International Journal of Nanomedicine. 2012;6003-9.

Finney DJ. A statistical treatment of the sigmoid response curve. Probit analysis. Cambridge University Press, London. 1971;633.

Abdelbaky AS, Abd El-Mageed TA, Babalghith AO, Selim S, Mohamed AM. Green synthesis and characterization of ZnO nanoparticles using Pelargonium odoratissimum (L.) aqueous leaf extract and their antioxidant, antibacterial and anti-inflammatory activities. Antioxidants. 2022;11(8):1444.

Ashokan AP, Paulpandi M, Dinesh D, Murugan K, Vadivalagan C, Benelli G. Toxicity on dengue mosquito vectors through Myristica fragrans-synthesized zinc oxide nanorods, and their cytotoxic effects on liver cancer cells (HepG2). Journal of Cluster Science. 2017 Jan;28: 205-26.

Lakshmi NJ, Anandakumar S, Sampathkumar V. Green synthesis and characterisation of ZnO nanoparticles from Manihot esculenta (cassava) peel and their antibacterial study. Global Nest Journal, 2023;25(3):56-62.

Jamdagni P, Khatri P, Rana JS. Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthes arbor-tristis and their antifungal activity. Journal of King Saud University-Science. 2018;30(2):168-75.

Das RK, Gogoi N, Bora U. Green synthesis of gold nanoparticles using Nyctanthes arbortristis flower extract. Bioprocess and Biosystems Engineering. 2011;34:615-9.

Awwad AM, Salem NM, Abdeen AO. Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. International Journal of Industrial Chemistry. 2013;4:1-6.

Umamaheswari A, Prabu SL, John SA, Puratchikody A. Green synthesis of zinc oxide nanoparticles using leaf extracts of Raphanus sativus var. Longipinnatus and evaluation of their anticancer property in A549 cell lines. Biotechnology Reports. 2021;29:e00595.

Venkateasan A, Prabakaran R, Sujatha V. Phytoextract-mediated synthesis of zinc oxide nanoparticles using aqueous leaves extract of Ipomoea pes-caprae (L). R. br revealing its biological properties and photocatalytic activity. Nanotechnology for Environmental Engineering. 2017;2:1-5.

Chaudhuri SK, Malodia L. Biosynthesis of zinc oxide nanoparticles using leaf extract of Calotropis gigantea: characterization and its evaluation on tree seedling growth in nursery stage. Applied Nanoscience. 2017;7(8):501-12.

Chithiga A, Manimegalai K. Biosynthesis of zinc oxide nanoparticles using Indigofera tinctoria and their efficacy against dengue vector, Aedes aegypti (Diptera: Culicidae). Experimental Parasitology. 2023;249: 108513.

Jayachandran A, Aswathy TR, Nair AS. Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract. Biochemistry and Biophysics Reports. 2021;26:100995.

Abdullah FH, Bakar NA, Bakar MA. Comparative study of chemically synthesized and low temperature bio-inspired Musa acuminata peel extract mediated zinc oxide nanoparticles for enhanced visible-photocatalytic degradation of organic contaminants in wastewater treatment. Journal of Hazardous Materials. 2021 Mar 15;406: 124779.

Pillai AM, Sivasankarapillai VS, Rahdar A, Joseph J, Sadeghfar F, Rajesh K, Kyzas GZ. Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. Journal of Molecular Structure. 2020;1211:128107.

Amuthavalli P, Hwang JS, Dahms HU, Wang L, Anitha J, Vasanthakumaran M, et al. Zinc oxide nanoparticles using plant Lawsonia inermis and their mosquitocidal, antimicrobial, anticancer applications showing moderate side effects. Scientific Reports. 2021 Apr 23;11(1):8837.

Chinnathambi A, Alharbi SA, Lavarti R, Jhanani GK, On-Uma R, Jutamas K, Anupong W. Larvicidal and pupicidal activity of phyto-synthesized zinc oxide nanoparticles against dengue vector aedes aegypti. Environmental Research. 2023; 216:114574.

Manna B, Aditya G, Banerjee S. Vulnerability of the mosquito larvae to the guppies (Poecilia reticulata) in the presence of alternative preys. J Vector Borne Dis. 2008;5(3):200-6.

Seng CM, Setha T, Nealon J, Socheat D, Chantha N, Nathan MB. Community-based use of the larvivorous fish Poecilia reticulata to control the dengue vector Aedes aegypti in domestic water storage containers in rural Cambodia. Journal of Vector Ecology. 2008;33(1):139-44.

Anogwih JA, Makanjuola WA. Predator-Prey Density of Poecilia reticulata (guppy) under laboratory investigation. The Zoologist. 2010;8:47-51.

Ghosh SK, Chakaravarthy P, Panch SR, Krishnappa P, Tiwari S, Ojha VP, Dash AP. Comparative efficacy of two poeciliid fish in indoor cement tanks against chikungunya vector Aedes aegypti in villages in Karnataka, India. BMC Public Health. 2011;11:1-8.

Warbanski ML, Marques P, Frauendorf TC, Phillip DA, El‐Sabaawi RW. Implications of guppy (Poecilia reticulata) life‐history phenotype for mosquito control. Ecology and Evolution. 2017;7(10):3324-34.

Patil CD, Borase HP, Patil SV, Salunkhe RB, Salunke BK. Larvicidal activity of silver nanoparticles synthesized using Pergularia daemia plant latex against Aedes aegypti and Anopheles stephensi and nontarget fish Poecillia reticulata. Parasitology Research. 2012;111:555-62.

Ghdeeb NJ, Hussain NA. Antimicrobial activity of ZnO Nanoparticles prepared using a green synthesis approach. Nano Biomedicine and Engineering. 2023;15(1): 14-20.

Hsueh YH, Ke WJ, Hsieh CT, Lin KS, Tzou DY, Chiang CL. ZnO nanoparticles affect Bacillus subtilis cell growth and biofilm formation. PloS One. 2015;10(6): e0128457.

Mastanaiah J, Prabhavathi NB, Varaprasad B. In vitro antibacterial activity of leaf extracts of Lawsonia Inermis. International Journal of PharmTech Research. 2011;3(2):1045-9.