UTTAR PRADESH JOURNAL OF ZOOLOGY

For millennia, silver has been known to have powerful antibacterial effects. Topical silver dressings are now commonly used to treat infections in burns, open wounds, and persistent ulcers. Because pathogenic organisms evolve on a daily basis as a result of mutation and antibiotic resistance, the manufacture and investigation of nanoparticles for use in antibacterial garments, burn ointments, and medical device coatings is an important industrial topic of nanoscience. This is the first study on the synthesis of silver nanoparticles from leaf extract of the medicinal plant Barleria noctiflora. Through the use of FT-IR, UV-visible spectrum analysis, XRD analysis, SEM EDAX, and antimicrobial potential, the bioreduced nanoparticles were discovered and verified. The greatest absorption peak in the UV-visible spectrum examination was seen at 480 nm for the silver nanoparticles. Twelve distinct peaks were identified in the FTIR spectra of biogenic AgNPs, located at 3127.21, 1624.74, 1400.35, 1155.96, 1021.11, 906.70, 825.31, 718.73, 669.69, 538.93, 457.21, and 417.49 cm-1. Peaks at 2θ values of 10.545, 19.000, 21.137, 22.033, 23.370, 26.691, 31.507, and 33.747, corresponding heights of 23.40, 235.26, 750.78, 357.58, 335.93, 171.19, 186.87, and 115.38cts, respectively, indicated by XRD analysis that the silver particles generated in our experiments were nanocrystals. The silver nanoparticles were tested with "d" spacing values of 8.38940, 4.67087, 4.20328, 4.03422, 3.80651, 3.33986, 2.83949, and 2.65601. SEM spectral analysis revealed oval AgNPs with a size range of 20 m. Metallic nanoparticles generated from Barleria noctiflora were tested for antibacterial activity against gram-positive and gram-negative pathogens such as E. Coli, Staphylococcus aureus, and the fungi Aspergillus niger and Candida albicans using the disc diffusion method. Surprisingly, metallic Ag nanoparticles had strong inhibitory zones against the selected illnesses.