Effect of Pimpinella tirupatiensis Extract on Brain Free Radical Toxicity in STZ Induced Diabetic Rats

PDF

Published: 2023-11-21

DOI: 10.56557/upjoz/2023/v44i233772

Page: 116-123


D. Veera Nagendra Kumar *

Department of Zoology, Government College for Men (A), Kadapa, Andhra Pradesh, India.

C. Venkata Krishnaiah

Department of Zoology, Government Degree College, Puttur, A.P., India.

V. Gurumurthy

Department of Zoology, Government Degree College for Women, Madanapalle, A.P., India.

G. Seethamma

Department of Zoology, KVR Government College for Women (A), Kurnool, A.P., India.

M. Rama Mohan

Department of Zoology, SV College of Arts & Computer Sciences, Proddatur, AP., India.

C. Narasimha Rao

Department of Zoology, Government Degree College, Mydukur, A.P., India.

*Author to whom correspondence should be addressed.


Abstract

The objective of this study was to investigate the effects of Pimpinella tirupatiensis on oxidative stress markers in different regions of the brain (cerebral cortex, cerebellum, hippocampus, and Pons medulla) in rats with diabetes. Diabetes was induced in wistar rats by injecting them with STZ (40 mg/kg). The rats were then administered Pimpinella tirupatiensis aqueous extract (750 mg/kg / b.w. /day) and glibenclamide (Glb) (20 mg/ kg / b.w. /day) orally for 30 days. After 4 weeks of high blood sugar, the activity of antioxidant enzymes was measured in both the diabetic and control groups. Diabetes can worsen nerve damage and cause oxidative damage due to high blood sugar levels. The diabetic rats displayed a significant decrease in levels of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) and reduced glutathione. However, when ginger was administered orally, it enhanced the activity of antioxidant enzymes in the diabetic rats. This suggests that Pimpinella tirupatiensis has a protective effect on the brain by enhancing antioxidant defense mechanisms in diabetic rats. Furthermore, it has a neuroprotective effect by enhancing the brain's antioxidant defense mechanisms and thereby attenuating the progression of hyperglycemia and some complications caused by diabetes in the rat brain.

Keywords: Diabetes, Pimpinella tirupatiensis, SOD, GPx, glutathione, glibenclamide


How to Cite

Kumar, D. V. N., Krishnaiah, C. V., Gurumurthy, V., Seethamma, G., Mohan, M. R., & Rao, C. N. (2023). Effect of Pimpinella tirupatiensis Extract on Brain Free Radical Toxicity in STZ Induced Diabetic Rats. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(23), 116–123. https://doi.org/10.56557/upjoz/2023/v44i233772

Downloads

Download data is not yet available.

References

IDF Diabetes Atlas Ninth Edition (Internet); 2019. (Cited 2020 May 18). Available:https://www.diabetesatlas.org/upload/resources/material/20200302_133351_IDFATLAS9e-final-web.pdf

Mahmoud AM, Ahmed OM, Ashour MB, Abdel-Moneim A. In vivo and in vitro antidiabetic effects of citrus flavonoids; A study on the mechanism of action. International Journal of Diabetes in Developing Countries. 2015;35(3):216-222.

Brands AM, Kessels RP, de Haan EH, et al. Cerebral dysfunction in type 1 diabetes: Effects of insulin, vascular risk factors and blood-glucose levels. Eur J Pharmacol. 2004;490(1–3):159–168.

Allen KV, Frier BM, Strachan MW. The relation-ship between type 2 diabetes and cognitive dysfunction: longitudinal studies and their methodological limitations. Eur J Pharmacol. 2004;490(1–3):169–175.

Ceriello A. New insights on oxidative stress and diabetic complications may lead to a causal antioxidant therapy. Diabetes Care. 2003;26:1589–1596.

Rahman K. Studies on free radicals, antioxidants, and co-factors. Clinical Interventions in Aging. 2007;2:219–236.

Doungue HT, Kengne APN, Kuate D. Neuroprotective effect and antioxidant activity of Passiflora edulis fruit flavonoid fraction, aqueous extract, and juice in aluminum chloride-induced Alzheimer’s disease rats. Nutrire. 2018;43:23. 3.

Mastrocola R, Restivo F, Vercellinatto I, et al. Oxidative and nitrosative stress in brain mito-chondria of diabetic rats. J Endocrinol. 2005;187(1):37–44.

Upwar N, Patel R, Waseem N, Mahobia NK. Hypoglycemic effect of methanolic extract of Berberis Aristata DC stem on normal and streptozotocin induced diabetic rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2011;3:0975-1491.

Ajiboye BO, Oyinloye BE, Agboinghale PE, et al. Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats. Pharm Bio. 2019;57: 604–611.

Vedavathi S, Mrudula V, Sudhakar A. Tribal Medicine of Chittoor District, AP, Herbal Folklore Research Centre, Tirupati. 1997;110-111.

Rajendra Kumar Reddy P, Jayarama Reddy S. Elemental Concentrations in Medicinally Important Leafy Materials Chemosphere. 1997;34:2193-2212.

Sudarsanam G, Reddy KB, Nagaraju N. Somatic embryogenesis in Pimpinella Tirupatiensis Bal. and Subr, an endangered medicinal plant of Tirumala Hills. Int. J. Pharmacog. 1995;33:52-66.

Misra HP, Fridowich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. K. Biol Chem. 1972;247:3170-3175.

Aebi H. Catalase. Methods Enzymol. 1984;105:125-126.

Flohe, Gunzler WA. Glutathione peroxidase. Methods Enzymes. 1984;105: 115-121.

Carlberg I, Mannervik B. Glutathione reduction. In: Methods in Enzymologyed., Colowick, S.P. and Kalpan, N.O. Academic Press in Orlando. 1985;113:484-499.

Aker boom TPM, Sies H. Assay of glutathione, glutathione disulphide and glutathione mixed disulphides in biological samples. Methods in Enzymology. 1981; 77:373–382.

Tiwari BK, Pandey KB, Abidi AB, et al. Markers of oxidative stress during diabetes mellitus. J Biomark. 2013;378790.

Siddiqui MR, Taha A, Moorthy K, Hussain E, Basir SF, Baquer NZ. Amelioration of altered antioxidants status and membrane linked functions by vanadium and Trigonella in alloxan diabetic rat brains. J. Biosci. 2005;30(4):483–49.

Mary A Okesola, Basiru O Ajiboye, Babatunji E Oyinloye, Olukemi A Osukoya, Ofogho S Owero-ozeze, Lisa I Ekakitie, Abidemi P Kappo. Effect of Solanum macrocarpon Linn leaf aqueous extract on the brain of an alloxan induced rat model of diabetes. Journal of International Medical Research. 2020;48(5):1–10.

Streck EL, Vieira PS, Wannmacher CMD, Dutra Filho CS, Wajner M, Wyse ATS. In vitro effect of homocysteine on some parameters of oxidative stress in rat hippocampus, Metabolic Brain Disease. 2003;18(2):147–154.

Celi P. The role of oxidative stress in small ruminants’ health and production. R Bras Zootec. 2010;39:348–363.

Oluwafemi Adeleke Ojo, Mary Abiola Okesola, Lisa Ilobekemen Ekakitie, Basiru Olaitan Ajiboye, Babatunji Emmanuel Oyinloye, Precious Eseose Agboinghale, et al. Gongronema latifolium Benth. leaf extract attenuates diabetes-induced neuropathy via inhibition of cognitive, oxidative stress and inflammatory response. Journal of the Science of Food and Agriculture. 2020;100(12):4504-4511.

Dai N, Zou Y, Zhu L, et al. Antioxidant properties of proanthocyanidins attenuate carbon tetrachloride (CCl4)-induced steatosis and liver injury in rats via CYP2E1 regulation. J Med Food. 2014; 17:663–669.

Mansouri E, Panahi M, Ghaffari M, Ghorbani A. Effects of grape seed proanthocyanidin extract on oxidative stress induced by diabetes in rat kidney, Iranian Biomedical Journal. 2011;15(3): 100–106.

Pugazhenthi S, Nesterova A, Jambal P, et al. Oxidative stress-mediated down-regulation of bcl-2 promoter in hippocampal neurons, Journal of Neurochemistry. 2003;84(5):982–996.

Vijay Kumar M, Govindarajan R, Rao GMM, Rao Ch V, Shirwaikar A, Mehrotra S, Oushpangadan P. Action of Hygophiola auriculata against streptozotocin induced oxidative stress, Journal of Ethnopharmacology. 2006;104:356-361.

Simonian NA, Coyle TJ. Oxidative stress in neurodegenerative diseases, Annual Review of Pharmacology and Toxicology. 1996;36(1):83–106.

Dincer Y, Akcay T, Alademir Z, Ilkova H. Effect of oxidative stress on glutathione pathway in red blood cells from patients with insulin dependent diabetes mellitus. Metabolism. 2002;51:1360-1362.

Kondeti Ramudu Shanmugam, Korivi Mallikarjuna, Nishanth Kesireddy, Kesireddy Sathyavelu Reddy. Neuroprotective effect of ginger on anti-oxidant enzymes in streptozotocin-induced diabetic rats. Food and Chemical Toxicology. 2011;49:893–897

Veera Nagendra Kumar D, Shanmugam KR, Ch Ramakrishna, Narasimhulu G, Lavanya T, Rajeswara Reddy S, et al. Neuroprotective effect of Pimpinella tirupatiensis tuberous root aqueous extract on brain antioxidant status in stz-induced diabetic rats. World Journal of Pharmaceutical Research. 2015;4(10): 2424-2435.