Potential Nephrotoxic Effect of UV-328 and Its Possible Salvage by Dimethoxy Curcumin in Zebrafish


Published: 2023-12-29

DOI: 10.56557/upjoz/2023/v44i243841

Page: 287-295

S. Senthilmurugan

Department of Zoology, Annamalai University, Annamalainagar, India.

R. Prinitha

Department of Zoology, Annamalai University, Annamalainagar, India.

P. Vijayan

Department of Zoology, Annamalai University, Annamalainagar, India.

S. Miltonprabu *

Department of Zoology, University of Madras, Chennai, India.

*Author to whom correspondence should be addressed.


The occurrence of Benzotriazole UV Stabilizer-328 (UV-328) in different environmental and natural systems is of fast regular concern these days. In this current paper, we assessed the renotoxicity of UV-328 in zebrafish kidney tissues to know the chore of oxidative devilry in kidney and to recuperate the renotoxicity utilizing Dimethoxy curcumin (DiMC) supplementation. Grown-up zebrafish were exposed 55µg/L of UV-328 and DiMC supplemented through diet at 50mg/kg BW. Close to the completion of 28 days, renal tissues were examined for the responses of oxidative pressure, antioxidant status and histopathological changes. The results demonstrated that antioxidant enzymes such as, Glutathione (GSH) levels and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) were all reduced in zebra fish kidneys treated with UV-328. Renal malondialdehyde (MDA) levels was brought prominently up in the UV-328 treated fish. All of the altered variables significantly returned to near-normal levels in the DiMC-supplemented group. Histopathological lesions, viz., hypertrophied glomerulus, cytoplasmic and nuclear degeneration, cytoplasmic vacuolization, degeneration of renal tubules were seen in UV-328 treated kidney of zebrafish which were actually reinforced in the DiMC treated fish. From our outcomes, it has been proposed that even at the low-level convergence of UV-328 exposure is malicious and to provoke oxidative insult, cell reinforcement exhaustion and kidney neurotic devilry in zebrafish and remediation with DiMC has been ended up being the better choice to conquer the harmful oppression prompted by UV-328.

Keywords: UV-328, oxidative stress, antioxidants, histopathology, zebra fish

How to Cite

Senthilmurugan, S., Prinitha , R., Vijayan , P., & Miltonprabu, S. (2023). Potential Nephrotoxic Effect of UV-328 and Its Possible Salvage by Dimethoxy Curcumin in Zebrafish. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44(24), 287–295. https://doi.org/10.56557/upjoz/2023/v44i243841


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Vimalkumar K, Arun E, Krishna-Kumar S, Poopal RK, Nikhil NP, Subramanian A, Babu-Rajendran R. Occurrence of triclocarban and benzotriazole ultraviolet stabilizers in water, sediment, and fish from Indian rivers. Sci. Total Environ. 2018; 625:1351-1360 Available:https://doi.org/10.1016/j.scitotenv.2018.01.042.

Liang X, Adamovsky O, Souders CL, Martyniuk CJ. Biological effects of the benzotriazole ultraviolet stabilizers UV-234 and UV-320 in early-staged zebrafish (Danio rerio). Environmental Pollution. 2019;245:272-281.


Denghel H, Leibold E, Göen T. Oxidative phase I metabolism of the UV absorber 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV 328) in an in vitro model with human kidney microsomes. Toxicol. In vitro. 2019;60:313-322. Available:https://doi.org/10.1016/j.tiv.2019.06.012

Zhang J, Huang Y, Pei Y, Wang Y, Li M, Chen H, Liang X, Martyniuk CJ. Biotransformation, metabolic response, and toxicity of UV-234 and UV-326 in larval zebrafish (Danio rerio). Environment International. 2023;174:107896 Available:https://doi.org/10.1016/j.envint.2023.107896.

Burgos-Aceves MA, Cohen A, Smith Y, Faggio C. MicroRNAs and their role on fish oxidative stress during xenobiotic environmental exposures. Ecotoxicol. Environ. Saf. 2018;148:995-1000 Available:https://doi.org/10.1016/j.ecoenv.2017.12.001

Velanganni S, Sivakumar P, Miltonprabu S. Impact of environmentally relevant concentration of benzophenone-3 on antioxidant enzymes, oxidative stress markers and morphology of gills in Danio rerio (Hamilton). GSC Biological and Pharmaceutical Sciences. 2021;14(03): 189-196.

Dai YJ, Jia YF, Chen N, Bian WB, Li QK, Ma YB, Chen YL, Pei DS. Zebrafish as a model system to study toxicology, Environ. Toxicol. Chem. 2014;33:11-17.

Manouchehr Teymouri, Nastaran Barati, Matteo Pirro, Amirhosein Sahebkar. Biological and pharmacological evaluation of dimethoxycurcumin: A metabolically stable curcumin analogue with a promising therapeutic potential. J Cell.Physiol. 2018; 233:124-140. Available:https://doi.org/10.1002/jcp.25749

Velanganni S, Miltonprabu S. Effect of benzophenone-3 at the environmentally relevant concentration on the kidney of Zebra fish Danio rerio (Hamilton). International Journal of Ecology and Environmental Sciences. 2021;(04):640-646.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979;4:582(1):67-78. DOI: 10.1016/0304-4165(79)90289- PMID: 760819

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 1974;7:469-474.


Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-126. Available:https://doi.org/10.1016/ S0076-6879(84)05016-3

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 1974;249:7130-7139.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science. 1973;179:588-590 Available:https://doi.org/10.1126/science.179.4073.588

Devasagayam TP, Boloor KK, Ramasarma T. Methods for estimating lipid peroxidation: an analysis of merits and demerits. Indian J. Biochem. Biophys. 2003;40:300-308.

Lowry OH, Rosebrough NJ, Farr AL, Randall RI. Protein measurement with folin phenol reagent. J. Biol. Chem. 1951; 193:265-275.

Pearse AGE. Histochemistry. Theoretical and applied, 3rd Eds vol-I. J.A. Churchill Ltd, London. 1968;13-102.

Roberts RJ. The pathophysiology and systematic pathology of teleosts, and laboratory methods. Fish Pathology, 1st Ed 67. Bailliere Tindall, London. 1978;235-246.

Humason GL. Animal tissue techniques. 4th Ed. W.H. Freeman and Company, San Francisco, California. 1979;661.

Miltonprabu S, Shagirtha K, Senthilmurugan S. Biochemical perturbations and metabolic derangements induced by benzophenone-3 at environmentally relevant concentration in the liver of Danio rerio. Uttar Pradesh Journal of Zoology. 2023;44(18):1-10 DOI: 10.56557/UPJOZ/2023/v44i18359

Chen QL, Sun YL, Liu ZH, Li YW. Sex-dependent effects of sub acute mercuric chloride exposure on histology, antioxidant status and immune-related gene expression in the liver of adult zebrafish (Danio rerio). Chemosphere. 2017;188:1-9 Available:https://doi.org/10.1016/j.chemosphere.2017.08.148

Zhang C, Wang J, Zhang S, Zhu L, Du Z, Wang J. Acute and sub chronic toxicity of pyraclostrobin in zebrafish (Danio rerio). Chemosphere. 2017;188:510-516. Available:https://doi. org/10.1016/j.chemosphere.2017.09.025.

Liu H, Xu H, Jiang Y, Hao S, Gong F, Mu H, et al. Preparation, Characterization, In vivo pharmacokinetics, and biodistribution of polymeric micellar dimethoxycurcumin for tumor targeting. Int. J. Nanomedicine. 2015;10:6395-6410. DOI: 10.2147/IJN.S91961

Jayakumar S, Patwardhan RS, Pal D, Sharma D, Sandur SK. Dimethoxy-curcumin, a metabolically stable analogue of curcumin enhances the radiosensitivity of cancer cells: Possible Involvement of ROS and thioredoxin reductase. Biochem. Biophysical Res. Commun. 2016;478:446-454. DOI: 10.1016/j.bbrc.2016.06.144