Histopathology, Isolation and Molecular Detection of Pasturella multocida from Sheep in Gadag Region of Karnataka, India
Chandrashekar KM *
Department of Veterinary Microbiology, Veterinary College, Gadag - 582101, KVAFSU, Bidar, India.
Shashidhar Ballari
Department of Veterinary Pathology, Veterinary College, Gadag - 582101, KVAFSU, Bidar, India.
Sanjeev Kumar M
Department of Veterinary Microbiology, Veterinary College, Gadag - 582101, KVAFSU, Bidar, India.
Akshata S. Angadi
Department of Veterinary Pathology, Veterinary College, Gadag - 582101, KVAFSU, Bidar, India.
Pradeep Chawhan
Department of Veterinary Microbiology, Veterinary College, Gadag - 582101, KVAFSU, Bidar, India.
Shivaraj B M
Southern Regional Disease Diagnostic Laboratory (SRDDL), IAH&VB, Hebbal, Bengaluru - 560024, KVAFSU, Bidar, India.
*Author to whom correspondence should be addressed.
Abstract
Bacterial pneumonia is the most common respiratory problem in small ruminants. The heart blood and lung tissue samples from two dead sheep suffering from pneumonia at the age of six month were subjected for histopathology, bacterial isolation & identification and antibiogram. Acute fibrinous bronchopneumonia was observed at necropsy and histopatholgy. The isolates were identified as P. multocida based on cultural and biochemical properties, and additionally confirmed by species-specific PCR amplification of KMT1 (Multocida toxin hydrolase gene) gene with the amplicon of 460 bp. The antibiogram showed susceptibility to chloramphenicol, β-lactams, quinolones, fluoroquinolones and aminoglycosides. In this study, the occurrence of ovine pasteurellosis caused by P. multocida with acute fibrinous bronchopneumonia was recorded.
Keywords: Histopathology, KMT1gene, pasteurellosis, Pasteurella multocida