Genetic Diversity Analysis of Rhynocoris marginatus Fabricius Based on 18S Ribosomal RNA Gene (Heteroptera: Reduviidae)

T. Bharathi

Department of Zoology, TDA College, Kannirajapuram, Tamil Nadu- 623135, India.

Arul Baskar *

Department of Zoology, Saiva Bhanu Kshatriya College, Aruppukottai, Tamil Nadu- 626101, India.

*Author to whom correspondence should be addressed.


Abstract

The R. marginatus are essential components of ecosystem, but also important in the biological control of insect pest, infesting a varity of agro ecosystem and medicine. The present investigation was carried out in the insect molecular genetic variation of 18S Ribosomal RNA gene from R. marginatus. The study was represent by the reduviid insect R. marginatus nucleotide gene sequences were translate amino acid sequence and obtained hydropathy, Domain, Transmembranes of proteins were calculated. The multiple gene sequence alignment of in-silico translated amino acid sequence of the partial ribosomal genes protein of R. marginatus were generated and the phylogenetic relationships were observed.

Keywords: R. marginatus, nucleotide sequence, phylogeney, 18S RNA, genetic variation


How to Cite

Bharathi , T., & Baskar , A. (2024). Genetic Diversity Analysis of Rhynocoris marginatus Fabricius Based on 18S Ribosomal RNA Gene (Heteroptera: Reduviidae). UTTAR PRADESH JOURNAL OF ZOOLOGY, 45(10), 65–72. https://doi.org/10.56557/upjoz/2024/v45i104049

Downloads

Download data is not yet available.

References

Fritz GN, Conn J, Cockburn AS, Eawright J. Sequence analysis of ribosomal DNA internal transcribed spaces 2 from populations of Anopheles nunezyovari (Diptera: Culicidae). Molecular Biology and evolution.1994;11:406-416.

Ambrose E, Arockia l, Angeline KD. Intrageneric phylogenetics based on mitochondrial DNA variation among fifteen harpactorine assassin bugs with four ecotypes and three morphs (Hemiptera: Reduviidae: Harpactorinae). Zootaxa. 2014;3779(5):540-550.

Putshkov VG, Putskov PV. A catalogue of assassin bugs genera of the world (Heteroptera: Reduviidae). VINITI, Moskva. 1985;138.

Maldonado J. Systematic catalogue of the Reduviidae of the world (Insecta: Heteroptera). Caribbean Journal of Science special editions, University of Puerto Rico, Mayaguez. 1990;694.

Baskar A, Dhanasekaran KG, Thirumurugan AR, Vignesh and Prakash. B. Molecular genetic variation and phylogeny of genus Rhynocoris based Mitochondrial Cytochrome c Oxidase subunit I gene (Heteroptera: Reduviidae). Indian journal of Natural Sciences. 2014; 4(22):1-13.

Wolstenholme. Animal mitochondrial DNA structure and evolution. Int Rev. cyto; 1992.

Yu Nie, Yu- Thian Fu. Yu Zhang Yun ping Deng, Wei wang, Ya Tu and Guo –Hau liu., Highly rearranged mitochondrial genome in falcoli perurislice (Phthiroptera; Philoptera) from endangered eagles; 2021.

Paschallis N, Philip H, Irepan Savador-Martinoz, Maximillan J. Computtional discovery of hidden breakes in 28s ribosomal RNAs across eukaryotes nd consequences for RNA integrity number scientific reports. 2019;9;19477 Available:https//doi.org,10.1038/41598-019-55573-1.

Wosee CR. Bacterial evolution microbiol.Rev. 1987;51:221-271.

Winker S, Woese CR. A definition of the domains, Archaea, bacteria and eucarya, in terms of small subunit ribosomal RNA characteristics- syst. Appl.Microbiol. 1991; 14:305-310.

Avise JC, Arnold J, Ball RM, Berminghan E, Lamb T, Neigel J, Neigel JE, Reeb CA, Saunder NC. Intraspecific phylogeograpy the mitochondrial DNA bridge between population genetics and systematic. Annu, Rev.Ecol.syst. 1987;18:489-522.

Moritz C, Dowling TE, Brown WM. Evolution of Animal mitochondrial DNA relevance for population biology and systematic. Annu, Rev. Ecol. Syst. 1987; 269-292.

Arthur K, Maria K, Emeline I, Eric C, Julie P, Jerome C, Jerome M. Shot gun assembly of the assassin bug Brontostoma colossus mitochondrial genome (Heteroptera: Reduviidae). Gene. 2014;4c: 39959-11.

Jon MM, James R, Garey. and Jeffrey. WS. Ecdysozoan phylogeny and Bayesian inference: First use of nearly complete 28s and 18s rRNA gene sequences to classify the arthropods and their kin. Molecular phylogenetic and evolution. 2003;5:213-216.

Christians W, James B Munro. 2009. Molecular phylogeny of the assassin bugs (Hemiptera: Reduviidae), based on mitochondrial and nuclear ribosomal genes. Molecular phylogenetics & Evolution. 2009;53:287-299.

Yingqi L, Hu L, Wanzhi C. Revision of the Assassin Bug geneus Sigicoris Stat Nov Based on morphological study and molecular phylogeny (Heteroptera: Reduviidae: Peiraatinae). Insect- 2022; 13951.

Uday kumar M, Tasashi IT. Molecular characterization and phylogeny of Linguatala serrata (Pentastomida: Linguatulidae) based on the nuclear 18s rDNA and mitochondrial cytochrome C oxidase I gene. J. vet. Med . Sci. 2017;79 (2):398-402.

Gillespie JJ, Johntoon JS, Cannones JJ, Guttells RR. Characteristics of the nuclear (18s, 5.8s, 28s and 5s) and mitochondrial (12s and 5s) rRNA genes of Apis mellifera (Insecta: Hymenoptera) structure, organization, and retro transposable elements. Insect molecular biology. 2006; 15(5):657-686.

Anilkumar N, Ansu Kumari VR, Kundave Sukhdeep V, Hira R. Molecular insights into the population structure and haplotype network of Therila annulata based on the small sub unit ribosomal RNA (18S rRNA) gene. Infection, Genetics and Evolution. 2022;99:105252.

Eisuke H, Eiiti K. Phylogenetic analysis of the insect order odonata using 28s and 16s rDNA sequence; a comparison between data sets with different evolutionary rates. Entomological science. 2006;9:55-66.

Mahendran B, Ghosh SK, Kundu S. C. Molecular phylogeny of silk producing insects based on 16s ribosomal RNA and Cytochrome oxidase subunit I gene. Indian Acadamy of science. J. Genet. 2006; 85:31-38.

Guryev V, Makarevitch I, Blinov A. and Martin J. Phylogeny of genes Chirnomous (Diptera) inferred from DNA sequences of mitochondrial Cytochrome b and Cytochrome oxidase I. Mol. Phylogenet. Evol. 2001;19:9-21.

Austin JW, Szalanski Allen L, Kard Bradford M. Distribution and genetic variation of Reticlatitermes (Isoptera: Rhinotermitidae) in Oklahoma. Florid Entomological Society. 2004; 87(2):152-158.

Arunkumar Muralidhar Melta KP, Nagaraja J. Molecular Phylogeny of silkmoths reveals the origin of domesticated silkmoth, Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA. Molecular phylogenetics and Evolution. 2006;40:419-427.

Yogesh S., Shouche and Milind S patole. Sequence analysis of mitochondrial 16s ribosomal RNA gene fragment from seven mosquito species. J. Bio Sci. 2000;25: 361-366.

Eman M Abd-EIAzeem, Mohamed M Nada, Adel EA Amer, Rana HM Hussien. Isolation and identification of entomopathogenic fungi associated with the spiny bollworm and evaluation of their metabolites against the insect’s biological parameters. Egyptian journal of Agriculture Research. 2024; 102(1):155-163.