Effects of Feed Deprivation on the Biochemical Responses of Pangasianodon hypophthalmus

K. S. Charan *

Govt. of Gujarat, India.

Nagesh, T.S.

Department of FRM, WBUAFS, Kolkata, India.

Abraham, T.J.

Department of AAH, WBUAFS, Kolkata, India.

Bhanu Prakash Ch

Department of AQC, PVNRTVU, Hyderabad, India.

Sarita, K. Das

Department of FRM, WBUAFS, Kolkata, India.

*Author to whom correspondence should be addressed.


Abstract

The effects of feed deprivation, including feed restriction and starvation, were investigated on the biochemical responses of Pangasianodon hypophthalmus over a 28-day period. Juvenile P. hypophthalmus were divided into three groups: control (daily feeding), feed limited (fed every third day) and deprived (no feeding). Blood samples were collected weekly. Results showed a significant decrease (P<0.05) in total protein and calcium levels in fish subjected to feed restriction and starvation. Although glucose levels decreased, the difference was not statistically significant (P>0.05). Cortisol levels in feed-deprived fish showed significant variations (P<0.05) with an erratic trend. Notably, lactate dehydrogenase (LDH) and creatinine levels exhibited significant increases (P<0.05). Throughout the starvation period, alanine transaminase (ALT) and aspartate transaminase (AST) levels rose, yet compared to the control, their concentrations did not significantly differ (P>0.05). The study concludes that total protein, LDH, and calcium, among other biochemical markers, were particularly sensitive to hunger-induced stress in P. hypophthalmus. This kind of study already conducted on some freshwater catfishes (Mustafa S., Tripathi G.) and catfiahes.

Keywords: Stress biomarkers, Pangasianodon hypophthalmus, feed-deprivation stress, biochemical parameters, fish starvation, fish serum test


How to Cite

Charan, K. S., Nagesh, T.S., Abraham, T.J., Bhanu Prakash Ch, & Das , S. K. (2024). Effects of Feed Deprivation on the Biochemical Responses of Pangasianodon hypophthalmus. UTTAR PRADESH JOURNAL OF ZOOLOGY, 45(11), 9–18. https://doi.org/10.56557/upjoz/2024/v45i114066

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References

Singh AK, Lakra WS. Culture of Pangasianodon hypophthalmus into India: Impacts and present scenario. Pakistan Journal of Biological Sciences. 2012;15(1): 19.

Möck, A., & Peters, G. J. J. O. F. B. (1990). Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution. Journal of Fish Biology. 37(6): 873-885.

Das A, Nagesh TS, Das SK, Abraham TJ. Stress responses of Indian major carps cultured in the East Kolkata Wetland, West Bengal, India. Aquatic Research. 2021; 4(4):351-362.

Gillis TE, Ballantyne JS. The effects of starvation on plasma free amino acid and glucose concentrations in lake sturgeon. Journal of Fish Biology. 1996;49(6):1306-1316.

Chatzifotis S, Papadaki M, Despoti S, Roufidou C, Antonopoulou E. Effect of starvation and re-feeding on reproductive indices, body weight, plasma metabolites and oxidative enzymes of sea bass (Dicentrarchus labrax). Aquaculture. 2011; 316(1-4):53-59.

Prasad G, Priyanka GL. Effect of fruit rind extract of Garcinia gummi-gutta on haematology and plasma biochemistry of catfish Pangasianodon hypophthalmus. 2011;6(3):240-251.

Yaghobi M, Dorafshan S, Akhlaghi M, Paykan Heyrati F, Mahmoudi N. Immune responses and intestinal morphology of striped catfish, Pangasianodon hypophthalmus (S auvage, 1878), fed dietary nucleotides. Journal of Applied Ichthyology. 2015;31(1):83-87.

Navarro I, Gutiérrez J. Fasting and starvation. In Biochemistry and molecular biology of fishes. Elsevier. 1995;4:393-434.

Mommsen TP, Vijayan MM, Moon TW. Cortisol in teleosts: Dynamics, mechanisms of action, and metabolic regulation. Reviews in Fish Biology and Fisheries. 1999;9:211-268.

Heming TA, Paleczny EJ. Compositional changes in skin mucus and blood serum during starvation of trout. Aquaculture. 1987;66(3-4):265-273.

Machado CR, Garofaloj MAR, Roselino JES, Kettelhut IDC, Migliorini RH. Effects of starvation, refeeding, and insulin on energy-linked metabolic processes in catfish (Rhamdia hilarii) adapted to a carbohydrate-rich diet. General and Comparative Endocrinology. 1988;71(3): 429-437.

Barton BA, Schreck CB, Fowler LG. Fasting and diet content affect stress‐induced changes in plasma glucose and cortisol in juvenile Chinook salmon. The Progressive Fish‐Culturist. 1988;50(1): 16-22.

Farbridge KJ, Leatherland JF. Plasma growth hormone levels in fed and fasted rainbow trout (Oncorhynchus mykiss) are decreased following handling stress. Fish Physiology and Biochemistry. 1992;10:67-73.

Borah S, Yadav RNS. Biochemical and haematological responses to starvation in an air breathing fresh water teleost Heteropneustes fossilis (Bloch). Indian J. Fish. 1996;43(3):307-311.

Friedrich M, Stepanowska K. Effect of starvation on nutritive value of carp (Cyprinus carpio L.) and selected biochemical components of its blood. Acta Ichthyologica et Piscatoria. 2001;31(2):29-36.

Soltanian S, Adloo MN, Hafeziyeh M, Ghadimi N. Effect of β-glucan on cold-stress resistance of striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878); 2014.

Ortiz RM, Wade CE, Ortiz CL. Effects of prolonged fasting on plasma cortisol and TH in postweaned northern elephant seal pups. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2001;280(3):R790-R795.

Pottinger TG, Rand-Weaver M, Sumpter JP. Overwinter fasting and re-feeding in rainbow trout: plasma growth hormone and cortisol levels in relation to energy mobilisation. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2003;136(3):403-417.

Costas B, Aragão C, Ruiz-Jarabo I, Vargas-Chacoff L, Arjona FJ, Dinis MT, Conceição LE. Feed deprivation in Senegalese sole (Solea senegalensis Kaup, 1858) juveniles: Effects on blood plasma metabolites and free amino acid levels. Fish Physiology and Biochemistry. 2011;37:495-504.

Caruso G, Denaro MG, Caruso R, Genovese L, Mancari F, Maricchiolo G. Short fasting and refeeding in red porgy (Pagrus pagrus, Linnaeus 1758): Response of some haematological, biochemical and non specific immune parameters. Marine Environmental Research. 2012;81:18-25.

Park IS, Hur JW, Choi JW. Hematological responses, survival, and respiratory exchange in the olive flounder, Paralichthys olivaceus, during starvation. Asian-Australasian Journal of Animal Sciences. 2012;25(9):1276.

Jung SH, Sim DS, Park MS, Jo Q, Kim Y. Effects of formalin on haematological and blood chemistry in olive flounder, Paralichthys olivaceus (Temminck et Schlegel). Aquaculture Research. 2003; 34(14):1269-1275.

Sheridan MA, Mommsen TP. Effects of nutritional state on in vivo lipid and carbohydrate metabolism of coho salmon, Oncorhynchus kisutch. General and Comparative Endocrinology. 1991;81(3): 473-483.

Tripathi G, Verma P. Starvation-induced impairment of metabolism in a freshwater catfish. Journal of Natural Sciences. 2003; 58:446–451

Love RM. The chemical biology of fishes. Vol.1. Acedamic press, New York. 1970;542.

Harper HA, Rodwell VW, Mayer PA. Review of physiological chemistry, seventeen ed. Lange Medical Publication, California. 1978;19-80.

Mohsen A, Momdouh AA, Maaly MA. Use of live Baker’s yeast, Saccharomyces cerevisiae, in practical diet to enhance the growth performance of Galilee Tilapia, Sarotherodon galilaeus L., and its resistance to environmental copper toxicity. Journal of the World Aquaculture Society. 2010;41:214-223.

Yamawaki K, Hashimoto W, Fujii K, Koyama J, Ikeda Y, Ozaki H. Hemochemical changes in carp Cyprinuscarpio exposed to low cadmium concentration. Nippon Suisan Gakkaishi Bull. 1986;52:459- 466.

Olojo EAA, Abass AA, Olurin KB, Mbaka G. The potential use of certain protein metabolism parameters as biomarkers of heavy metal (lead) stress in the African cat fish, Clarias Gariepinus. Agricultural Journal. 2012;7(5):316-322.

Young SJ, Dowman AA, Cowell DC. The detection of pentachlorophenol by its inhibitory effectiveness on lactate dehydrogenase of rabbit muscle and Bovine Heart. Pesticide Biochemistry and Physiology. 1999;64(1):1-8.

De Coen WM, Janssen CR, Segner H. The use of biomarkers in Daphnia magna toxicity testing V. In vivo alterations in the carbohydrate metabolism of Daphnia magna exposed to sublethal concentrations of mercury and lindane. Ecotoxicology and Environmental Safety. 2001;48(3):223-234.

Ramesh M, Sankaran M, Veera-Gowtham V, Poopal RK. Hematological, biochemical and enzymological responses in an Indian major carp Labeo rohita induced by sublethal concentration of waterborne selenite exposure. Chemico-Biological Interactions. 2014;207:67-73.

Chatterjee N, Pal AK, Das T, Mohammed MS, Sarma K, Venkateshwarlu G, Mukherjee SC. Secondary stress responses in Indian major carps Labeo rohita (Hamilton), Catla catla (Hamilton) and Cirrhinus mrigala (Hamilton) fry to increasing packing densities. Aquaculture Research. 2006;37(5):472-476.

Das PC, Ayyappan S, Jena JK, Das BK. Acute toxicity of ammonia and its sub‐lethal effects on selected haematological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton). Aquaculture Research. 2004;35(2):134-143.

Tkachenko H, Kurhaluk N, Grudniewska J. Effects of chloramine-T exposure on oxidative stress biomarkers and liver biochemistry of rainbow trout, Oncorhynchus mykiss (Walbaum), brown trout, Salmo trutta (L.), and grayling, Thymallus thymallus (L.). Fisheries and Aquatic Life. 2013;21(1):41-51.

Hadi A, Shokr A, Alwan S. Effects of aluminum on the biochemical parameters of fresh waterfish Tilapia zillii. J. Sci. Appl. 2009;3(1):33-41.

Osman AG, Koutb M, Sayed AEDH. Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet–A radiation on African catfish Clarias gariepinus (Burchell, 1822). Journal of Photochemistry and Photobiology B: Biology. 2010;99(1):1-8.

Kulkarni R, Barad V. Haematological and blood biochemical changes in the fresh water fish, Notopterus notopterus (Pallas) exposed to acidic medium. International Letters of Natural Sciences. 2015;45.

Thangavel P, Sumathiral K, Karthikeyan S, Ramaswamy M. Endocrine response of the freshwater teleost, Sarotherodon mossambicus (Peters) to dimecron exposure. Chemosphere. 2005;61(8): 1083-1092.

Congleton JL, Wagner T. Blood‐chemistry indicators of nutritional status in juvenile salmonids. Journal of Fish Biology. 2006; 69(2):473-490.

Hoseini SM, Ghelichpour M. Effects of pre-sampling fasting on serum characteristics of common carp (Cyprinus carpio L.). International Journal of Aquatic Biology. 2013;1(1):6-13.

Das SK, Nagesh TS, Das A, Abraham TJ, Vishwanath TS. Stress Mitigating and Growth-Enhancing Effect of Dietary Vitamin E in Indian Major Carps Cultured in East Kolkata Wetlands, India. In Proceedings of the Zoological Society. New Delhi: Springer India. 2022;75(2):208-220.

Gabriel UU, George AOI, Plasma enzymes in Clariasgariepinusexposed to chronic levels of roundup (glyphosate). Environ Ecol. 2005;23(2):271–276.

Mustafa S. Changes in biochemical composition in starving catfish heteropneustes fossilis. Japanese Journal of Ichthyology. 1983;29(4):416- 420.