Molecular Detection of Antibiotics Profile of Citrobacter freundii Isolated from Burn Infection
Amaal M. Khudaier
Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq.
Nawras M. Al-Khafaji *
Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq.
Azal A. Al-Rubaeaee
AL-Safwa University College, Karbala, Iraq.
*Author to whom correspondence should be addressed.
Abstract
Fifty samples were taken for this study from burn victims receiving care at the Al-Hussien medical city between February and October 2021. In order to isolate Citrobacter freundii, two swabs were taken: one for culturing and the other for direct examination.
Only12 (4.1%) of the 50 samples were isolated from burn and wound infections at the molecular level and 12 (4.1%) from culture. The results isolation and laboratory diagnosis as well as biochemical test approved that there is only 12 isolates belong to C.freundii confirmed by molecular detection by specific primers.
Burn and wound is one of the rare causes of infection. Associated with sub sequent urinary tract infection and burn and wound infection.
Three antibiotics were employed at the molecular level , the results showed that 10 samples were resistant to the (Tet b) gene, all samples were 100% sensitive to the PIP gene, and all samples (100% resistant to the erna gene).
Keywords: Citrobacter freundii, antibiotics pattern, qrnb, Erna, Tetb, pip