Micronucleus Assay and Hematological Parameters Study due to Presence of Heavy Metals in Four Different Species of Freshwater Fishes of River Mahanadi Cuttack Odisha
Subhashri Priyadarsini
*
Giet University, Gunupur, Odisha, India.
T. Gayatri
Giet University, Gunupur, Odisha, India.
Satya Narayan Sahoo
Department of Zoology, NIALI College, Cuttack, Odisha, India.
*Author to whom correspondence should be addressed.
Abstract
Mahanadi river water is highly polluted. Alkalinity, hardness, pH of water has changed in the last few years. Heavy metals like Pb , Fe, Cu, Mn, K quantity increases which causes hematological and genetic damage to the aquatic ecosystem. Fish are the best biomarkers to find out the threats towards aquatic ecosystem. Water pollution causes hematological damage as well as genetic damage and formation of micronucleus. Water sample analysis occurs by evaporation method for extraction of heavy metals. Fish species like Labeo rohita, Catla catla, Cirrhinus mrigala, Cirrihinus reba collected from the several stations of Cuttack 20.440N 85.880E to 20.460N -85.890E area and for Hematological study blood was collected by puncturing the caudal vein. Micronucleus assays done by use of Giemsa staining method as a result it shows formation of micronucleus and DNA damage which indicate the presence of high level of pollutants in water of River Mahanadi Cuttack Odisha.
Keywords: Heavy metals (Pb; Fe; Mn; Cu; K), evaporation method, aquatic ecosystem, hematological study, micronucleus, DNA damage, giemsa stain