Study on the Genotoxic Effect of Copper Sulphate in the Spotted Snakehead Fish Channa Punctatus (Bloch, 1793)

Subhashree Jagyanseni *

Department of Zoology, GIET University, Gunupur, Odisha, India.

Somanath Sahoo

GIET University, Gunupur, Odisha, India.

Satya Narayan Sahoo

Department of Zoology, Niali College, Niali, Cuttack, Odisha, India.

*Author to whom correspondence should be addressed.


The genotoxic effects of a herbicide containing CuSO4 were assessed using the micronucleus assay in Channa punctatus. The study involved intraperitoneal administration of three different doses (1.0, 3.0, and 5.0 mg/kg body weight) and exposure to varying concentrations of copper sulphate (15, 25, and 35 ppm) in laboratory aquaria. Peripheral blood smears stained with 15 to 20% Giemsa (pH=7.0) were examined. Apart from micronuclei, the herbicide induced other nuclear and cytoplasmic abnormalities. The findings suggest a direct impact of increasing CuSO4 concentration on the biological samples of Channa punctatus. This fish species is commonly found in freshwater habitats like ponds, ditches, wetlands, and rice fields, especially in Odisha, India. India is crucial for maintaining aquatic biodiversity. The study highlights the potential detrimental effects of improper use of CuSO4 containing pesticides in agriculture on Channa punctatus and emphasizes the need for careful management of such chemicals to protect aquatic ecosystems.

Keywords: Channa punctatus, copper sulphate, Micronucleus (MN) Assay, genotoxic potential, peripheral blood smear

How to Cite

Jagyanseni, S., Sahoo, S., & Sahoo, S. N. (2024). Study on the Genotoxic Effect of Copper Sulphate in the Spotted Snakehead Fish Channa Punctatus (Bloch, 1793). UTTAR PRADESH JOURNAL OF ZOOLOGY, 45(12), 274–280.


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Banerjee SM, Mitra E. Preliminary observations on the use of copper sulphate to control submerged aquatic weeds in alkaline waters. Indian journal of fisheries. 1954;204-216.

Babu GR, Rao PV. Heavy metal ion toxicity in the freshwater gastropod snail host, Lymnaea luteola. Indian Journal of Physiology and Pharmacology. 1982;26 (2):141-146,

Luoma SN, Rainbow PS. Sources and cycles of trace metals. In: Metal Contamination in Aquatic Environments: Science and Lateral Management. Cambridge University Press, Cambridge. 2008a;47-66.

Livingstone D R. Oxidative stress in aquatic organisms in relation to pollution and agriculture. Revue de Medicine Veterinaire. 2003;154:427– 430.

Shukor Y, Baharom NA, Rahman FA, Abdullah MP, Shamaan NA, Syed MA. Development of heavy metals enzymatic-based assay using papain. Analytica Chemica Acta. 2006;566:283-289.

Shukor MY, Masdor N, Baharom NA, Jamal JA, Abdullah MPA, Shamaan NA, Syed MA . An inhibitive determination method for heavy metals using bromelain, a cysteine protease. Appl Biochem Biotechnol. 2008;144(3):283-291.

Singh D, Nath K, Sharma YK, Trivedi SP. Hepatotoxic effect of Cu (II) in fresh water fish Channa punctatus: A histopathological study. Res. Environ. Life Sci. 2008;1(1):13-16.

Di Toro DM, Allen HE, Bergman HL, Meyer JS. Biotic ligand model of the acute toxicity of metals.1.Technical basis. Environ Toxicol Chem. 2001;20(10):2383-2396.

Santore RC, Di Toro DM , Paquin PR, Allen HE, Meyer JS. Biotic ligand model of the acute toxicity of metals. II. Application to acute copper toxicity in freshwater fish and daphnia. Environ Toxicol Chem. 2001;20 (10):2397-2402.

Jagyanseni S, Mishra S, Sahoo S N. A Study on genotoxic potential of acephate in Clarias batrachus. Journal for Research in Applied Sciences and Biotechnology. 2023;2(1):22-25.

Al-Sabti K. Clastogenic effects of five carcinogenic mutagenic chemicals on the cells of the common carp Cyprinus carpio L. Com Biochem Physiol. 1986;85 (C):5-9.

Das R K, Nanda N K. Induction of micronuclei in peripheral erythrocytes of fish Heteropneustes fossilis by mitomycin C and paper mill effluent. Mutation research. 1986;175: 67-61.

Okpokwasili GC. Contributions of industrial pollution and environmental degradation on the emergence of communicable and non-communicable diseases. A paper presented at the5thAnnualNational Conference of Society for Occupational Safety and Environmental Health (SOSEH); 2009.

Lyne TB, Bickham JW, Lamb T, Gibbons JW. The application of bioassays in risk assessment of environmental pollution. Risk Anal.1992;12(3):361-365.

Germain A, Perron F,Van Coillie R. PAHs in the Environment: Fate, Ecotoxicity and Regulations, Environment Canada, Conservation and Protection, Quebec Region, Montreal,Que; 1993.

Vuković-Gačić B, Kolarević S, Sunjog K, Tomović J, KneževićVukčević J, Paunović M, Gačić Z. Comparative response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. Hydrobiologia. 2013;735:221–231.

Rocco L, Peluso C, Stingo V. Micronucleus test and comet assay for the evaluation of zebrafish genomic damage induced by erythromycin and lincomycin. Environ Toxicol. 2012;27:598–604.

Sunjog K, Gačič Z, Kolarevič S, Visnjić-Jeftić Ž, Jarić I, Knežević-Vukčević J, Vuković-Gačić B, Lenhardt M. Heavy metal accumulation and the genotoxicity in Barbel (Barbus barbus) as the indicators of the Danube River pollution. Sci World J. 2012;1–6.

Kolarević S, Knežević-Vukčević J, Paunović M, Tomović J, Gačić Z, Vuković-Gačić B. The anthropogenic impact on water quality in river Danube in Serbia: microbiological analysis and genotoxicity monitoring. Arch Biol Sci. 2011;63:1209–1217.

Van der Oost R, Beyer J, Vermeulen N P E. Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environ Toxicol Pharma. 2003;13: 57–149.

Xin L, Wang J, Guo S, Wu Y, Li X, Deng H, Kuang D, Xiao W, Wu T, Guo H. Organic extracts of coke oven emissions can induce genetic damage in metabolically competent HepG2 cells. Environ Toxicol Pharmacol. 2014;37:946–953

Szefer P, Szefer K, Skwarzec B. Distribution of trace metals in some representative fauna of the Southern Baltic. Mar Poll Bull. 1990;21:60–62.

Visn-Jeftic Z, Jaric I, Jovanovic L, Skoric S, Smederavac-Lalic M, Niksevic M, Lenhardt M. Heavy metal and trace element accumulation in muscle liver and gills of the Pontic shad (Alosa immaculate Bennet 1835) from the Danube River (Serbia). Microchem J. 2010;95:341–344.

Arslan O Ç, Parlak H. Micronucleus test good biomarker for determination of genetic changes in aquatic organism. J Aquat Pollut Toxicol. 2017;1(3): 18-23.

Guecheva T, Henriques JAP , Erdtmann B . Genotoxic Effects of Copper Sulphate in Freshwater Planarian In vivo, Studied with Single- Cell Gel Test (Comet Assay). Mutation Research. 2001; 497:19-27.

Yadav KK, Trivedi SP. Sublethal exposure of heavy metals induces micronuclei in fish, Channa punctata. Chemosphere. 2009;77(11):1495-1500.

Kousar S, Javed M. Studies on induction of nuclear abnormalities in peripheral blood erythrocytes of fish exposed to copper. Turk J Fish Aquat Sci. 2015;15(4): 879-886.

Talapatra SN, Banerjee SK. Detection of micronucleus and abnormal nucleus in erythrocytes from the gill and kidney of Labeo bata cultivated in sewage-fed fish farms. Food Chem Toxicol. 2007;45(2): 210-215.

Singh D, Nath K, Trivedi SP, Sharma YK. Impact of copper on haematological profile of fresh water fish Channa punctatus. J. Environ. Biol. 2008;29(2): 253-257.

Jagyanseni S, Sahoo S, Sahoo SN. Genotoxic and haematological effect of commonly used insecticide on fish Heteropneustes fossilis. International Journal of Modern Pharmaceutical Research. 2023;7(7):27-29.

Kashyap A, Kumar V. Histopathological Analysis of Hexavalent Chromium Toxicity to Ovary and Testis of Freshwater Food Fish, Channa punctatus (Bloch. 1793). Asian J. Fish. Aqu. Res. 2023;23(4): 18-25. Available: [Accessed on 2024 May 22]

Akan JC, Salwa M, Yikala BS, Chellube ZM. Study on the Distribution of Heavy Metals in Different Tissues of Fishes from River Benue in Vinikilang, Adamawa State, Nigeria. Curr. J. Appl. Sci. Technol. 2012;2 (4):311-33. Available: [Accessed on 2024 May 22].

Idrees N, Sarah R, Tabassum B, Abd_Allah EF. Evaluation of some heavy metals toxicity in Channa punctatus and riverine water of Kosi in Rampur, Uttar Pradesh, India. Saudi Journal of Biological Sciences. 2020;27(5): 1191-4.