Adenia hondala-derived Biopolymer Nanoparticles Cause G2/M Cell Cycle Arrest in Breast Cancer Cells
Pradeepa Varadharajaperumal *
Department of Biotechnology, Sri Kaliswari College, Sivakasi 626130. Tamil Nadu, India.
Sujatha Muthuswamy
Department of Biotechnology, Sri Kaliswari College, Sivakasi 626130. Tamil Nadu, India.
Devi Pothagar
Department of Biotechnology, Sri Kaliswari College, Sivakasi 626130. Tamil Nadu, India.
Mareeswari Ganesan
Department of Biotechnology, Sri Kaliswari College, Sivakasi 626130. Tamil Nadu, India.
Amutha Santhanam
National Centre for Nanoscience and Nanotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Silver nanoparticles (AgNPs) are a type of nanomaterials known for their antimicrobial and disinfectant properties due to their detrimental effects on targeted cells. This study investigated the delivery of a novel therapeutic system based on chitosan-coated silver nanoparticles (Ch-Ag NPs) for Tamoxifen to MCF-7 human breast cancer cells. The Ag NPs were synthesized from Adenia hondala tuber extract and coated with chitosan to form Ch-Ag NPs. Tamoxifen was loaded onto these nanoparticles to create Tam-Ch-AgNPs, functioning as a drug delivery system. Apoptosis induction was confirmed through annexin V staining and analysis of mitochondrial membrane potential. Cell cycle analysis showed G2/M phase arrest in Tam-Ch-AgNPs-treated cells, indicating apoptosis induction. The inhibition of cancer cell growth by Tam-Ch-AgNPs suggests potential for developing eco-friendly therapeutic agents in nano medicine, particularly for cancer treatment, prompting further research in this field.
Keywords: Nanoparticles, cell cycle, Adenia hondala, tamoxifen, breast cancer