REDESCRIPTION OF TREMATODE PARASITES FOUND IN CHANNA PUNCTATA BLOCH, 1793 WITH AN EMPHASIS ON SCANNING ELECTRON MICROSCOPY STUDY
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Published
Apr 5, 2021
Page:
10-24
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I. KUNDU
Department of Zoology, Barasat Government College, Barasat, Kolkata-700124, West Bengal, India.
P. K. BANDYOPADHYAY
Department of Zoology, Parasitology Laboratory, University of Kalyani, Kalyani-741235, West Bengal, India.
D. R. MANDAL
South Asian Forum for Environment, Kolkata, West Bengal, India.
Abstract
The paper presents a systematic survey of helminth parasites found in Channa punctata from various districts (Nadia, North 24 Paraganas and Hooghly) in West Bengal. Mainly two species of parasites Euclinostomum heterostomum and Clinostomum complanatum have been recorded during the study. Data on their morphology and site of infection are provided. Morphological studies with light and scanning electron microscopic studies as well as molecular studies have revealed some additional morphological details. New features which were observed with scanning electron microscopy in Euclinostomum heterostomum were aspinous and smooth tegument surface, transversely folded areas, absence of spines on both the suckers and presence of crates. Dome shaped papillae with microridges and micropores on the surface of suckers was also observed. Similarly aspinous suckers with papillae on the floor of the ventral sucker was reported in Clinostomum complanatum. All the helminthes have been briefly described and illustrated. The study was done on economically important fishes to establish database of various types of infection caused by helminth parasites.
Keywords:
Channa punctata, helminth, scanning electron microscopy, taxonomy
Article Details
How to Cite
KUNDU, I., BANDYOPADHYAY, P. K., & MANDAL, D. R. (2021). REDESCRIPTION OF TREMATODE PARASITES FOUND IN CHANNA PUNCTATA BLOCH, 1793 WITH AN EMPHASIS ON SCANNING ELECTRON MICROSCOPY STUDY. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(7), 10-24. Retrieved from http://mbimph.com/index.php/UPJOZ/article/view/2034
Section
Original Research Article
References
Zaidi DA, Khan D. Life history of Euclinostomum minutes Bhutta and Khan Digenea: Euclinostomidae. Pak J Zool. 1975;7: 161–176.
Jhansilakshmibai K, Madhavi R. Euclinostomum heterostomum (Rudolphi, 1809) (Trematoda): Life-cycle, growth and development of the metacercaria and adult. Syst Parasitol. 1997;38: 51–64.
Echi PC, Eyo JE, Okafor FC. Co-parasitism and morphometrics of three Clinostomatids (Digenea:Clinostomatidae) in Sarotherodon melanotheron from a tropical freshwater lake. Anim Res Int. 2009;6: 982–986.
Montes MM, Plaul SE, Croci Y, Waldbillig M, Ferrari W, Topa E, et al. Pathology associated with three new Clinostomum metacercariae from Argentina with morphological and DNA barcode identification. J Helminthol. 2020; 94(e148):1–11.
Available:https://doi.org/10.1017/S0022149X20000292
Purivirojkul W, Sumontha M. Euclinostomum heterostomum (Rudolphi, 1809) metacercarial infection in three Osphronemid fish species. Walailak Journal. 2013;10:97–102.
Bullard SA, Overstreet RM. Digeneans as enemies of fishes. In: Eiras JC, Segner H, Wahli T, Kapoor BG (eds) Fish diseases. Science Publishers, Enfield, Jersey. 2008;2: 817–976.
Kanev I, Radev V, Fried B. Family Clinostomidae Luehe, 1901. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, Wallingford, UK. 2002;1:113–120.
Locke SA, Caffara M, Marcogliese DJ, Fioravanti ML. A large-scale molecular survey of Clinostomum (Digenea: Clinostomidae). Zool Scr. 2015;44: 203–217.
Pérez-Ponce de León G, García-Varela M, Pinacho-Pinacho CD, Sereno-Uribe AL, Poulin R. Species delimitation in trematodesusing DNA sequences: Middle-American Clinostomum as a case study. Parasitology. 2016;143:1773–1789
Caffara M, Locke SA, Echi PC, Halajian A, Benini D, Luus Powell WJ, et al. A morphological and molecular study of Clinostomid metacercariae from African fish with a redescription of Clinostomum tilapiae. Parasitology. 2017;144:1519–1529.
Caffara M, Locke SA, Gustinelli A, Marcogliese DJ, Fioravanti ML. Morphological and molecular differentiation of Clinostomum complanatum and Clinostomum marginatum (Digenea: Clinostomidae) metacercariae and adults. J Parasitol. 2011;97:884–891.
Yamaguti S. Systema helminthum. The digenetic trematodes of vertebrates Part I and II, New York. 1958;I:1141–1143.
Eisenback JD. A comparison of techniques useful for preparing nematodes for scanning electron microscopy. J Nematol. 1986;18:479-487.
Travassos L. Sur la systematique de la familie des clinostomidae Luhe, 1901. Comptes rendus des Seances de a Societ6 de Comt Rend Soc Biol P Biologie et de ses Fihales Paris. 1928;98:643–644.
Ukoli FMA. On Euclinostomum heterostomum (Rudolphi, 1809). J Helminthol. 1966;40:227–234.
DOI: https://doi.org/10.1017/S0022149X00034210
Dennis EA, Sharp M. Morphology of Euclinostomum heterostomum (Rudolphi, 1809) (Trematoda: Clinostomatidae) from Bulbicus ibis. J Helminthol. 1973;47: 17-25
Suanyuk N, Mankhakhet S, Soliman H, Saleh M, El-Matbouli M. Euclinostomum heterostomum infection in guppies Poecilia reticulata cultured in southern Thailand. Dis Aquat Organ 2013;104:121–127.
DOI: 10.3354/dao02595
Nadakavukaren MJ, Nollen PM. A scanning electron microscopic investigation of the outer surfaces of Gorgoderina attenuate. Int J Parasitol. 1975;5:591- 595.
Bakke A. Functional morphology and surface topography of Leucochloridium sp. (Digenea) revealed by scanning electron microscope. Z Parasitenkd. 1976;51:115-128.
DOI:doi.org/10.1007/BF00380533
Bakke TA, Bailey RE. Phyllodistomum umblae (Fabricius) (Digenea, Gorgoderidae) from British Columbia samonids: A description based on light and scanning electron microscopy. Can J Zool. 1987;65:1703-1712.
DOI: doi.org/10.1139/z87-263
Otubanjo OA. Scanning electron microscopic studies of the body surface and external genitalia of a dicrocoeliid trematode, Concinnum epomopis Sandground 1973. Z Parasitenkd. 1985;71:495-504.
DOI: doi.org/10.1007/BF00928352
Barber KE, Caira JN. Investigation of the life cycle and adult morphology of the avian blood fluke Austrobilharzia variglandis (Trematoda: Schistosomatidae) from Connecticut. J Parasitol. 1995;1(4):584-92.
Miller FH, Tulloch GS, Kuntz RE. Scanning electron microscopy of integumental surface of Schistosoma mansoni. J Parasitol. 1972;58: 693-698.
DOI:doi.org/10.2307/3278293
Emarus DA. The host parasite interface of strigeoid IX.A probe and transmission electron microscopic study of the tegument of Diplostomum phonixi Faust 1918. Parasitology. 1970;61:35- 41.
Bennet CE. Surface features and sensatory structures and movement of freshly excysted juvenile Fasciola hepatica L. J Parasitol. 1975a;61:886- 891.
DOI:doi.org/10.2307/3279229
Vankara AP, Mani G, Vijayalakshmi C. A report on various digenetic metacercariae from the freshwater fishes of River Godavari, Rajahmundry. J Parasit Dis. 2011;35:177-185.
DOI: doi.org/10.1007/s12639-011-0041-8
Athokpam VD, Jyrwa DB, Tandon V. Utilizing ribosomal DNA gene marker regions to characterize the metacercariae (Trematoda: Digenea) parasitizing piscine intermediate hosts in Manipur, Northeast India. J Parasit Dis. 2014;40:330–338.
Caffara M, Locke SA, Cristanini C, Davidovich N, Markovich MP, Fioravanti ML. A combined morphometric and molecular approach to identifying metacercariae of Euclinostomum heterostomum (Digenea: Clinostomidae). J Parasitol. 2016;102:239–248.
DOI: doi.org/10.1645/15-823
Senapin S, Phiwsaiya K, Laosinchai P, Kowasupat C, Ruenwongsa P, Panijpan B. Phylogenetic Analysis of Parasitic Trematodes of the Genus Euclinostomum found in Trichopsis and Betta Fish. J Parasitol. 2014;100(3):368-371.
DOI:doi.org/10.1645/13-285.1
Leidy J. A synopsis of Entozoa and some of their ectocongeners observed by the author. Proc Acad Nat. Sci Philadelphia. 1856;8:42-58.
Simsek E, Yildirim A, Yilmaz E, Inci A, Duzlu O, Onder Z, et al. Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey. Parasitol Res. 2018;117(7):2117-2124.
DOI: 10.1007/s00436-018-5898-3
Gholami Z, Mobedi I, Esmaeili HR, Kia EB. Occurrenceof Clinostomumcomplanatum in Aphanius dispar (Actinoptrygii: Cyprinodontidae) collected from Mehran River, Hormuzgan Province, South of Iran. Asian Pac J Trop Biomed. 2011;1(3):189–192.
DOI: 10.1016/S2221-1691(11)60025-7
Ngamniyom A, Manaboon M, Panyarachun B. Thai Medaka, Oryzias minutillus Smith, 1945(Beloniformes: Adrianichthyidae): A new host species of Clinostomum complanatum metacercariae (Digenea: Clinostomatidea) and the surface topography by using SEM. Chiang Mai J Sci. 2012;39(3):540-544.
Scholz T, Vargus Vazquez J, Moravec F, Vikas C, Rodriguez E, Franco M. Metacerariae of trematodes of fishes from cenotes of the Yucatan Peninsula, Mexico. Folia Parasitol. 1995;42:173-192.
Marwan A, Mohammed TA. Scanning electron microscopy of Clinostomum metacercaria from Oreochromis niloticus from Assiut Egypt. Egypt J Exp Biol. 2003;5:70-77.
El-Naggar MM, Ibrahim HA, Hamada SF. Scanning and transmission electron microscope observations on the general body tegument and ventral pads of the digenean Orientocreadium batrachoides Tubangui 1931. Egyptian German Society of Zoology 10(D) Inver Zool Parasitol. 1993;245-264.
Abidi SMA, Ahmad M, Nizami WA, Hanna REB. Clinostomum complanatum: Tegumental surface: Changes during in-vivo development. Int J Parasitol. 1998;18: 433-439.
DOI:10.1016/0020-7519(88)90005-7
Font WF, Whittrock DD. Scanning electron microscopy of Leucochloridiomorpha constantiae during development from metacercaria to adult. J Parasitol. 1980;66: 955–964.
Voge M, Price Z, Bruckner DA. Changes in tegumental surface during development of Schistosoma mansoni. J Parasitol. 1978;64: 585–592.
DOI:doi.org/10.2307/3279937
Crabtree JE, Wilson RA. Schistosoma mansoni: A scanning electron microscope study of the developing schistosomulum. Parasitology. 1980;81:553–564.
DOI: doi.org/10.1017/S003118200006193X
Basch PF, Basch N. Schistosoma mansoni: scanning electron microscopy of schistosomula, adults and eggs grown in-vitro. Parasitology. 1982;85:333–338.
DOI: 10.1017/s0031182000055311
Jhansilakshmibai K, Madhavi R. Euclinostomum heterostomum (Rudolphi, 1809) (Trematoda): Life-cycle, growth and development of the metacercaria and adult. Syst Parasitol. 1997;38: 51–64.
Echi PC, Eyo JE, Okafor FC. Co-parasitism and morphometrics of three Clinostomatids (Digenea:Clinostomatidae) in Sarotherodon melanotheron from a tropical freshwater lake. Anim Res Int. 2009;6: 982–986.
Montes MM, Plaul SE, Croci Y, Waldbillig M, Ferrari W, Topa E, et al. Pathology associated with three new Clinostomum metacercariae from Argentina with morphological and DNA barcode identification. J Helminthol. 2020; 94(e148):1–11.
Available:https://doi.org/10.1017/S0022149X20000292
Purivirojkul W, Sumontha M. Euclinostomum heterostomum (Rudolphi, 1809) metacercarial infection in three Osphronemid fish species. Walailak Journal. 2013;10:97–102.
Bullard SA, Overstreet RM. Digeneans as enemies of fishes. In: Eiras JC, Segner H, Wahli T, Kapoor BG (eds) Fish diseases. Science Publishers, Enfield, Jersey. 2008;2: 817–976.
Kanev I, Radev V, Fried B. Family Clinostomidae Luehe, 1901. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, Wallingford, UK. 2002;1:113–120.
Locke SA, Caffara M, Marcogliese DJ, Fioravanti ML. A large-scale molecular survey of Clinostomum (Digenea: Clinostomidae). Zool Scr. 2015;44: 203–217.
Pérez-Ponce de León G, García-Varela M, Pinacho-Pinacho CD, Sereno-Uribe AL, Poulin R. Species delimitation in trematodesusing DNA sequences: Middle-American Clinostomum as a case study. Parasitology. 2016;143:1773–1789
Caffara M, Locke SA, Echi PC, Halajian A, Benini D, Luus Powell WJ, et al. A morphological and molecular study of Clinostomid metacercariae from African fish with a redescription of Clinostomum tilapiae. Parasitology. 2017;144:1519–1529.
Caffara M, Locke SA, Gustinelli A, Marcogliese DJ, Fioravanti ML. Morphological and molecular differentiation of Clinostomum complanatum and Clinostomum marginatum (Digenea: Clinostomidae) metacercariae and adults. J Parasitol. 2011;97:884–891.
Yamaguti S. Systema helminthum. The digenetic trematodes of vertebrates Part I and II, New York. 1958;I:1141–1143.
Eisenback JD. A comparison of techniques useful for preparing nematodes for scanning electron microscopy. J Nematol. 1986;18:479-487.
Travassos L. Sur la systematique de la familie des clinostomidae Luhe, 1901. Comptes rendus des Seances de a Societ6 de Comt Rend Soc Biol P Biologie et de ses Fihales Paris. 1928;98:643–644.
Ukoli FMA. On Euclinostomum heterostomum (Rudolphi, 1809). J Helminthol. 1966;40:227–234.
DOI: https://doi.org/10.1017/S0022149X00034210
Dennis EA, Sharp M. Morphology of Euclinostomum heterostomum (Rudolphi, 1809) (Trematoda: Clinostomatidae) from Bulbicus ibis. J Helminthol. 1973;47: 17-25
Suanyuk N, Mankhakhet S, Soliman H, Saleh M, El-Matbouli M. Euclinostomum heterostomum infection in guppies Poecilia reticulata cultured in southern Thailand. Dis Aquat Organ 2013;104:121–127.
DOI: 10.3354/dao02595
Nadakavukaren MJ, Nollen PM. A scanning electron microscopic investigation of the outer surfaces of Gorgoderina attenuate. Int J Parasitol. 1975;5:591- 595.
Bakke A. Functional morphology and surface topography of Leucochloridium sp. (Digenea) revealed by scanning electron microscope. Z Parasitenkd. 1976;51:115-128.
DOI:doi.org/10.1007/BF00380533
Bakke TA, Bailey RE. Phyllodistomum umblae (Fabricius) (Digenea, Gorgoderidae) from British Columbia samonids: A description based on light and scanning electron microscopy. Can J Zool. 1987;65:1703-1712.
DOI: doi.org/10.1139/z87-263
Otubanjo OA. Scanning electron microscopic studies of the body surface and external genitalia of a dicrocoeliid trematode, Concinnum epomopis Sandground 1973. Z Parasitenkd. 1985;71:495-504.
DOI: doi.org/10.1007/BF00928352
Barber KE, Caira JN. Investigation of the life cycle and adult morphology of the avian blood fluke Austrobilharzia variglandis (Trematoda: Schistosomatidae) from Connecticut. J Parasitol. 1995;1(4):584-92.
Miller FH, Tulloch GS, Kuntz RE. Scanning electron microscopy of integumental surface of Schistosoma mansoni. J Parasitol. 1972;58: 693-698.
DOI:doi.org/10.2307/3278293
Emarus DA. The host parasite interface of strigeoid IX.A probe and transmission electron microscopic study of the tegument of Diplostomum phonixi Faust 1918. Parasitology. 1970;61:35- 41.
Bennet CE. Surface features and sensatory structures and movement of freshly excysted juvenile Fasciola hepatica L. J Parasitol. 1975a;61:886- 891.
DOI:doi.org/10.2307/3279229
Vankara AP, Mani G, Vijayalakshmi C. A report on various digenetic metacercariae from the freshwater fishes of River Godavari, Rajahmundry. J Parasit Dis. 2011;35:177-185.
DOI: doi.org/10.1007/s12639-011-0041-8
Athokpam VD, Jyrwa DB, Tandon V. Utilizing ribosomal DNA gene marker regions to characterize the metacercariae (Trematoda: Digenea) parasitizing piscine intermediate hosts in Manipur, Northeast India. J Parasit Dis. 2014;40:330–338.
Caffara M, Locke SA, Cristanini C, Davidovich N, Markovich MP, Fioravanti ML. A combined morphometric and molecular approach to identifying metacercariae of Euclinostomum heterostomum (Digenea: Clinostomidae). J Parasitol. 2016;102:239–248.
DOI: doi.org/10.1645/15-823
Senapin S, Phiwsaiya K, Laosinchai P, Kowasupat C, Ruenwongsa P, Panijpan B. Phylogenetic Analysis of Parasitic Trematodes of the Genus Euclinostomum found in Trichopsis and Betta Fish. J Parasitol. 2014;100(3):368-371.
DOI:doi.org/10.1645/13-285.1
Leidy J. A synopsis of Entozoa and some of their ectocongeners observed by the author. Proc Acad Nat. Sci Philadelphia. 1856;8:42-58.
Simsek E, Yildirim A, Yilmaz E, Inci A, Duzlu O, Onder Z, et al. Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey. Parasitol Res. 2018;117(7):2117-2124.
DOI: 10.1007/s00436-018-5898-3
Gholami Z, Mobedi I, Esmaeili HR, Kia EB. Occurrenceof Clinostomumcomplanatum in Aphanius dispar (Actinoptrygii: Cyprinodontidae) collected from Mehran River, Hormuzgan Province, South of Iran. Asian Pac J Trop Biomed. 2011;1(3):189–192.
DOI: 10.1016/S2221-1691(11)60025-7
Ngamniyom A, Manaboon M, Panyarachun B. Thai Medaka, Oryzias minutillus Smith, 1945(Beloniformes: Adrianichthyidae): A new host species of Clinostomum complanatum metacercariae (Digenea: Clinostomatidea) and the surface topography by using SEM. Chiang Mai J Sci. 2012;39(3):540-544.
Scholz T, Vargus Vazquez J, Moravec F, Vikas C, Rodriguez E, Franco M. Metacerariae of trematodes of fishes from cenotes of the Yucatan Peninsula, Mexico. Folia Parasitol. 1995;42:173-192.
Marwan A, Mohammed TA. Scanning electron microscopy of Clinostomum metacercaria from Oreochromis niloticus from Assiut Egypt. Egypt J Exp Biol. 2003;5:70-77.
El-Naggar MM, Ibrahim HA, Hamada SF. Scanning and transmission electron microscope observations on the general body tegument and ventral pads of the digenean Orientocreadium batrachoides Tubangui 1931. Egyptian German Society of Zoology 10(D) Inver Zool Parasitol. 1993;245-264.
Abidi SMA, Ahmad M, Nizami WA, Hanna REB. Clinostomum complanatum: Tegumental surface: Changes during in-vivo development. Int J Parasitol. 1998;18: 433-439.
DOI:10.1016/0020-7519(88)90005-7
Font WF, Whittrock DD. Scanning electron microscopy of Leucochloridiomorpha constantiae during development from metacercaria to adult. J Parasitol. 1980;66: 955–964.
Voge M, Price Z, Bruckner DA. Changes in tegumental surface during development of Schistosoma mansoni. J Parasitol. 1978;64: 585–592.
DOI:doi.org/10.2307/3279937
Crabtree JE, Wilson RA. Schistosoma mansoni: A scanning electron microscope study of the developing schistosomulum. Parasitology. 1980;81:553–564.
DOI: doi.org/10.1017/S003118200006193X
Basch PF, Basch N. Schistosoma mansoni: scanning electron microscopy of schistosomula, adults and eggs grown in-vitro. Parasitology. 1982;85:333–338.
DOI: 10.1017/s0031182000055311