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The microbiological quality of ice cream during retail marketing mainly depends on the contamination during the product handling as well as efficiency and sanitary conditions during frozen storage. Many food poisoning cases associated with the consumption of ice creams have been reported. We studied the bacteriological profile of ice cream sold at retail outlets in Tiruchirappalli, a city in Tamilnadu State, India. Ice cream samples were collected from ice cream shops. Random sampling method was followed to collect different flavours of ice creams from the shops. A total of 10 samples were collected from two different localities, in sterile containers and transported to the laboratory and were processed immediately. High level coli form count, presence of Escherichia coli and Bacillus sp indicated faecal contamination of ice creams thereby suggesting possible risk of infection involved in the consumption of such food. Presence of Staphylococcus an enterotoxin producer may cause serious health problems. Among the isolates from samples tested, only Escherichia coli and Staphylococcus sp were significant potential pathogens. However, presence of other organisms (Bacillus) could be attributed to unhygienic conditions during preparation, handling and serving of ice creams. Based on the biochemical characterization, the isolate 1 was identified as Bacillus sp., the isolate 2 as E. coli sp., the isolate 3 as Staphylococcus sp. The molecular characterization revealed that the Bacillus sp, was identified as Bacillus subtilis, this was further submitted to NCBI Genbank to get Accession number. The Accession number was MH071337.1. Health education of the vendors and strict implementation of hygienic standards may help to reduce the contamination rates. The results suggested negligence such as poor sanitation during the preparation/or storage of ice cream. These included the observed dirty premises, used utensils and the use of bare hands in preparing the products.
Vica M, Glevitzky M, Dumitrel G, Popa M, Todoran A. Microbiological quality of ice cream produced in Alba county, Romania. Journal of Agroalimentary Processes and Technologies. 2010;16:19-23.
Aaku EN, Collison EK, Gashe BA, Mpuchane S: Microbiological quality of milk from two processing plants in Gaborone Botswana. Food Control. 2004;15:181-186.
Hankin L, Hanna JG. Quality of ice cream and ice milk. Bull Contecticuit Agric Exper Stat. 1984;818:6.
Yaman H, Elmali M, Ulukanli Z, Tuzcu M, Genctave K. Microbial quality of ice cream sold openly by retail outlets in Turkey. Revue. Med. Vet. 2006;157:457-462.
Dijuretic T, Wall PG, Nichols G. General outbreaks of infectious intestinal diseases associated with milk and dairy products in England and Wales 1992-1996. Communicable diseases Report CDR Review. 1997;7:41-45.
Dijuretic T, Wall PG. An outbreak of Salmonella infection from ice cream. The New Engl. J. Med. 1996;335:824.
Chugh K. Salmonella outbreak from ice cream. Indian Pediatr. 1996;33:976-977.
Verma GS. Bacteria quality of some ingredients used in the manufacture of ice Cream. Ind. J. Anim. Res. 1972;6:27-30.
Bathla JM, Rao YS. Microbial quality of ingredients in relation to that of Ice cream. J. Res. Sci. 1973;22:59-66.
Gomez A. Microbial content and hygienic conditions of ice cream sold in Leon Alimentaria. 1969;6:21-25.
Vasavada PC. Pathogenic Bacteria in milk: A Review Journal of Dairy Science. 1988;71: 2809-2816.
Tomislav P, Samarzija D, Zamberlin S. Psychro-trophic bacteria and milk and dairy products quality. Mljekarstvo. 2012;62(2):77-95.
Elahi ATM, Habib S, Rahman MM, Rahman GI, Bhuiyan MJU. Sanitary quality of commercially produced ice cream sold in the retail stores. Pakistan Journal of Nutrition. 2002;1(2):93-94.
Joshi DR, Shah PK, Manandhar S, Shama S, Banmali P. Microbial quality of ice cream sold in Kathmundu. Journal of Nepal Health Research Council. 2004;2(2):37-40.
Budová O, Baranová M, Lauková A, Różańska H, Rola JG. Hygiene of pasteurised milk depending on psychrotrophic microorganisms. Bulletin of the Veterinary Institute in Pulawy. 2002;46:325-329.
Nelapati S, Krishnaiah N, Kiranmayi B. Physiochemical and microbiological quality of ice creams sold in and around Greater Hyderabad Municipal Corporation. Journal of Veterinary Public Health. 2009;7(2):121– 124.
Warke R, Kamat A, Kamat M, Thomas P. Incidence of pathogenic psychrotrophs in ice creams sold in some retail outlets in Mumbai, India. Food Control. 2000;11:77- 83.
Marmur J. A procedure for the isolation of DNA from microorganisms. J. Mol. Biol. 1961; 3:208-218.
Lane DJ. 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics (Eds. E. Stackebrandt, M. Goodfellow) John Wiley and Sons, Chichester, United Kingdom. 2001;115- 175.
Food safety and standards Authority of India. Food safety and Standards (Food product standards and food additives) regulation, Part II: 498; 2011.
Kumar H, Wadhwa G, Palaha R, Gandhi R, Singh S. Microbiological quality analysis of ice creams sold by street hawkers: A case study of Jalandhar City, India. Internet J. Food Safety. 2011;13:164-169.
Ojokoh AO. Microbiological examination of ice cream sold in Akure, Pakistan. J. Nutr. 2006;5(6):536–538.
Beck E. Hygienic aspect of the production and marketing of soft ice cream (II). Backer and Konditor. 1973;27:297 298.
Trabulsi LR, Keller R, Tardelli Gromes TA. Typical and a typical enteropathogenic Escherichia coli. Emerging Infect. Dis. 2002; 8(5):508 513.
Anuranjini C, Sebastian Geethu B, Dhanashree B. Bacteriological analysis of ice creams from Mangalore, South India. Indian J. Med. Res. 2008;127:91 92.
Ahmed K, Shakoori AR. Vibrio cholerae El Tor, Ogawa 01 as the main etiological agent of the two major outbreaks of gastroenteritis in the Northern areas of Pakistan, Gilgit. Pak. J. Zool. 2002;34:73-80.
Jay JM. Modern Food Microbiology, 4th Edn., Champman and Hall Inc, New York; 1996.
Hobbs BC, Golbert JR. Food Poisoning and Food Hygiene. 4th Edn., Edward Arnold Limited, London. 1982;366.
Hennessy TW, Hedberg CW, Slutsker L, White KE, Besser-Wiek JM, Moen ME, Feldman J, Coleman WW, Edmonson LM, MacDonald KL. A national outbreak of Salmonella enteritidis infections from ice cream. The Investigation Team. Journal of Medicine. 1996; 334(20):1281-1286.
Bryan FL. Diseases transmitted by foods. In-The United States Centers for Disease Control, classification and summary. 2nd ed. Atlanta; 1982.
Prescott LM, Harley JP, Klein DN. Microbiology. 4th Edition, The McGraw-Hill Companies, Inc., New York. 1999;685.
Kambamanoli-Dimou A. Ice cream. In: Encyclopedia of Food Microbiology. Edited by Robinson, RK, Batt, CA & Patel, PD. Academic Press. 2000;2.