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Natural drugs play important and vital role in the modern medicine. They cure ailments which may not be treated by conventional medicine and exhibit many biological activities like antimicrobial. The purpose of our study is to screen phytochemicals and to determine antibacterial activity of hexane, diethyl ether, ethyl acetate and ethanol extracts of Tribulus terrestris leaves against five pathogens (Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium and Staphylococcus aureus). The extracts were prepared by cold maceration and the in vitro antimicrobial susceptibility testing was done using agar well diffusion method. The inhibition zones were measured for five concentrations (10 mg, 5 mg, 2.5 mg, 1.25 mg and 0.625 mg) of the extract. The phytochemical analysis revealed the presence of phenols, carbohydrates, flavones, saponins, steroids, alkaloids, proteins, quinones and tannins. In antibacterial assay, inhibition zones were detected in all extracts and in all concentrations. Large zones were formed in hexane and diethyl ether extracts. As the concentration of extract increased, the diameter of inhibition zones around the well also increased. Hence our results support the use of all the four extracts of Tribulus terrestris as a source of antibiotic substance for the possible treatment of human pathogenic organisms.

Plant extract, phytochemical, antimicrobial, agar well diffusion, zone of inhibition

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Jumaa S, Karaman R. Antibiotics. In: Commonly used drugs – uses, side effects, bioavailability and approaches to improve it. Ed: Karaman R, Nova Science Publishers. 2015;41-73. DOI: 10.13140/RG.2.1.5114.4804

Golkar Z, Bagasra O, Pace DG. Bacteriophage therapy: a potential solution for the antibiotic resistance crisis. J Infect Dev Ctries. 2014; 8(2):129-136. DOI: 10.3855/jidc.3573

Spellberg B, Gilbert DN. The future of antibiotics and resistance: a tribute to a career of leadership by John Bartlett. Clin Infect Dis. 2014;59(Suppl 2):S71–S75. DOI: 10.1093/cid/ciu392

Viswanathan VK. Off-label abuse of antibiotics by bacteria. Gut Microbes. 2014;5(1):3– 4. DOI: 10.4161/gmic.28027

Michael CA, Dominey-Howes D, Labbate M. The antimicrobial resistance crisis: causes, consequences, and management. Front Public Health. 2014;2:145. DOI: 10.3389/fpubh.2014.00145

Rossolini GM, Arena F, Pecile P, Pollini S. Update on the antibiotic resistance crisis. Curr Opin Pharmacol. 2014;18:56-60. DOI: 10.1016/j.coph.2014.09.006

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82. DOI: 10.1128/CMR.12.4.564

Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Nirali Prakashan Publisher, Pune. 2007;370.

Khare CP. Indian medicinal plants: An illustrated dictionary. Springer-Verlag Berlin Heidelberg, New York. 2007;669-671.

Samerdjieva IB, Zheljazkov VD. Chemical constituents, biological properties and uses of Tribulus terrestris: A review. Nat Prod Comm. 2019;14(8):1-26. Available:

Zhu W, Du Y, Meng H, Dong Y, Li L. A review of traditional pharmacological uses, phytochemistry and pharmacological activities of Tribulus terrestris. Chem Cent. 2017;11(1):60. DOI: 10.1186/s13065-017-0289-x

Oh H, Park SJ, Moon HD, Jun SH, Choi N, You Y. Tribulus terrestris inhibits caries-inducing properties of Streptococcus mutans. J Med Plant Res. 2011;5:6061-6. DOI: 10.5897/JMPR11.1008

Handa SS, Singh SP, Khanuja, Longo G, Rakesh DD. Extraction technologies for medicinal and aromatic plants, International centre for science and high technology, Trieste. 2008;22-24. Available:

Sivanandham V. Phytochemical techniques – a review. World Journal of Science and Research. 2015;1(2):80-91.

Magaldi S, Mata-Essayag S, Capriles CH, Perez C, Colella MT, Olaizola C, Ontiveros Y. Well-diffusion for antifungal susceptibility testing. Int J Infect Dis. 2004;8(1):39-45. DOI: 10.1016/j.ijid.2003.03.002

Brown WJ. National Committee for Clinical Laboratory Standards agar dilution susceptibility testing of anaerobic gram-negative bacteria. Antimicrob Agents Chemother. 1988;32(3):385–390. DOI: 10.1128/AAC.32.3.385

Laxminarayan R, Bhutta Z, Duse A, Jenkins P, O’Brien T, Okeke IN, et al. Drug resistance. In: Disease control priorities in developing countries. Jamison DT, Breman JG, Measham AR, Alleyne G, Claeson M, Evans D B, Jha P, Mills A, Musgrove P, Eds. Oxford University Press, New York. 2006;1031-51. Available:

Kayange N, Kamugisha E, Mwizamholya DL, Jeremiah S, Mshana SE. Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in a tertiary hospital, Mwanza-Tanzania. BMC Pediatr 2010;10: 39. DOI: 10.1186/1471-2431-10-39

Cheesman MJ, Ilanko A, Blonk B, Cock IE. Developing new antimicrobial therapies: are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution? Pharmacogn Rev. 2017;11(22):57–72. DOI: 10.4103/phrev.phrev_21_17

Cai L, Wu Y, Zhang J, Pei F, Xu Y, Xie S, Xu D. Steroidal saponins from Tribulus terrestris. Planta Med. 2001;67(2):196-198. DOI: 10.1055/s-2001-11650

Kumari M, Kumar P, Singh P. Safety evaluation of Tribulus terrestris on the male reproductive health of laboratory mouse. Int J Pharm Phytopharmacol Res. 2015;4(5):281-287. Available:

Kostova I, Dinchev D. Saponins in Tribulus terrestris – chemistry and bioactivity. Phytochem Rev. 2005;4(2-3):111-137. DOI: 10.1007/s11101-005-2833-x

Wu T, Shi L, Kuo S. Alkaloids and other constituents from Tribulus terrestris. Phytochem. 1999;50(8):1411-1415. Available:

Chu S, Qu W, Pang X, Sun B, Huang X. Effect of saponin from Tribulus terrestris on hyperlipidemia. Zhong Yao Cai. 2003;26(5): 341-344. PMID: 14535016

Sankeshwari RM, Ankola AV, Bhat K, Hullatti K. Soxhlet verusus cold maceration: Which method gives better antimicrobial activity to licorice extract against Streptococcus mutans. J Sci Soc. 2018;45(2):67-71. DOI: 10.4103/jss.JSS_27_18

Zhang Q, Lin L, Ye W. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20. DOI: 10.1186/s13020-018-0177-x

Abarca-Vargas R, Malacara CFP, Petricevich VL. Characterization of chemical compounds with antioxidant and cytotoxic activities in Bougainvillea x buttiana Holttum and Standl, (var. Rose) extracts. Antioxidants (Basel). 2016;5(4):45. DOI: 10.3390/antiox5040045

Truong H, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. J Food Qual. 2019;2019(1):1-9. Available:

Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pac J Trop Biomed. 2014;4(Suppl 1):359-367. DOI: 10.12980/APJTB.4.2014C1030

Silva APS, Silva LCN, Foseca C, Araujo JM, Correia MTS, Cavalcanti MS, Lima V. Antimicrobial activity and phytochemical analysis of organic extracts from Cleome spinosa Jaqc. Front Microbial. 2016;7: s963. DOI: 10.3389/fmicb.2016.00963

Boateng J, Diunase KN. Comparing the antibacterial and functional properties of Cameroonian and Manuka honeys for potential wound healing – have we come full cycle in dealing with antibiotic resistance? Molecules. 2015;20(9):16068-84. DOI: 10.3390/molecules200916068

Al-Bayati FA, Al-Mola HF. Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J Zhejiang Univ Sci B. 2008;9(2):154-159. DOI: 10.1631/jzus.B0720251

Kianbakht S, Jahaniani F. Evaluation of antibacterial activity of Tribulus terrestris L. growing in Iran. Iran J Pharm Ther. 2003; 2(1):22-24. Available:

Saravanasingh K, Ramamurthy M. Bioactivity studies of extracts from Tribulus terrestris. Int J Res Chem Pharm Sci. 2016; 3(1):40-49. Available:

Maheswari MSU, Rajendran R, Vijayalakshmi S. Pharmacological activity of different solvent extracts of Tribulus terrestris against multi drug resistant Staphylococcus aureus isolated from post-operative wound patients. Biosci Biotech Res Comm. 2017;10(4):752-763. Available:

Sasikala T, Prabakaran R, Sabitha S. Antimicrobial activities of Tribulus terrestris L. on selected pathogenic microorganisms. Int J Pharm Phytopharmacol Res. 2014;4(3):182-186. Available:

Soleimanpour S, Sedighinia FS, Afshar AS, Zarif R, Ghazvini K. Antibacterial activity of Tribulus terrestris and its synergistic effect with Capsella bursa-pastoris and Glycyrrhiza glabra against oral pathogens: an in-vitro study. Avicenna J Phytomed. 2015;5(3):210-7. PMID: 26101754

Batoei S, Mahboubi M, Yari R. Antibacterial activity of Tribulus terrestris methanol extract against clinical isolates of Escherichia coli. Herba Polonica. 2016;62(2):57-66. DOI:

Abdulqawi LNA, Quadri SA. Evaluation of Antibacterial and Antioxidant activities of Tribulus terrestris L. Fruits. Research Journal of Pharmacy and Technology. 2021;14(1):331-6. DOI: 10.5958/0974-360X.2021.00061.5

Baburao B, Rajyalakshmi G, Venkatesham A, Kiran G, Shyamsunder A, Gangarao B. Anti-inflammatory and antimicrobial activities of methanolic extract of Tribulus terrestris Linn plant. Int J Chem Sci. 2009;7(3):1867- 72.