LARVICIDAL CAPABILITY OF CHLOROFORM EXTRACTS OF Calophyllum inophyllum l. AND Cassia fistula L. ON Oryctes rhinoceros (LINN.) LARVAE in vitro

Main Article Content



Owing to the fact that the application of chemical pesticides cause adverse effects on beneficial organisms, pollute the environment and are detrimental to human health, plant based products are being tested for the control of variety of insect pests. The present study was carried out to test and compare the bioefficacy of chloroform solvent extracts of Cassia fistula and Calophylluminophyllum against Orychtesrhinocerous larvae The leaves of these two plants were dried, powdered and extracted in a Soxhlet apparatus using chloroform as the solvent. These leaf extracts exhibited dose-dependent mortality. The rate of mortality also increases with the increasing hours of exposure indicates more sensitivity.The plant extracts alters the Total Hemocyte Count and Differential hemocyte count.  The increased mortality  may be due to its antifeedant activity or may be due to its altering the physiological activity and endocrine glands which secretes regulatory hormone.Of these two plants C. inophyllum is more effective than C. fistula.Thus from the work it is concluded C. fistula and C. inophyllum showed varying degree of toxicity and can be used in Integrated Pest Management of O.rhinocerous  and minimise use of chemical insecticides.

Cassia fistula, Calophylluminophyllum, Orychtesrhinocerous, hemocyteinsecticides

Article Details

How to Cite
JINCY, T., & RENUGA, F. B. (2021). LARVICIDAL CAPABILITY OF CHLOROFORM EXTRACTS OF Calophyllum inophyllum l. AND Cassia fistula L. ON Oryctes rhinoceros (LINN.) LARVAE in vitro. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(24), 495-502. Retrieved from
Original Research Article


Sreeletha C, Geetha PR.Pesticidal effects of Clerodendroninfortunatum on the fat body of Oryctes rhinoceros (Linn.) male. Journal of Biopesticides. 2011;4 (1):13 – 17.

Mehta KS, Patyal SK, Rana RS, Sharma KC. Ecofriendly techniques for the management of Helicoverpaarmigera(Hubner) in tomato. Journal of Biopesticide.2010;3(1):296-303.

Mukherjee U, Singh HN. Eco-friendly approaches tomanage Diamond- Back moth, Plutellaxylostella(L.) in Cauliflower. Journal of Applied Zoological Research. 2006;17(1):57-60.

Nakatani M, Abd El-Galeil SAM, Kuruwaki J, Okamura H, Iwagawa T, Doe M.Antifeedant ring B and D opened limonoids from Khayasenegalensis. Journal of Natural Products. 2001;64:1263-1265.

Samidurai K, Jebanesan A, Saravana Kumar A, Govindarajan M, Pushpanathan T. Larvicidal, ovicidal and repellent activities of Pemphisacidulaforst against filarial and dengue vector mosquitoes. Academic Journal of Entomology. 2009;2(2): 62- 66.

Santi SR. Antifeedant compounds in nyamplung seed oil (CalophylluminophyllumL), Jurnal Kimia.2014;8(2):226-230.

KadarusmanRatnaPujiAstuti, Widayani Neneng S, DonoDanar, HidayatYusup, Sunarto Toto, Hartati Sri, Maharani Rani. Toxicity of seed oil of azadirachtaindica, calophylluminophyllum and their mixture against crocidolomiapavonana. larvae Research Journal of Chemistry and Environment.2018;22(II):267- 272.

Govindarajan M, Jebanesan A. Pushpanathan T.Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes. Parasitol Res. 2008;102:289–292.

Duraipandiyan V, Ignacimuthu S, Gabriel Paulraj M. Antifeedant and larvicidal activities of Rhein isolated from the flowers of Cassia fistula L. Saudi Journal of Biological Sciences. 2011;18:129–133.

Li YZ, Li LZ, Hua HM, Li ZG, Liu MS. Studies on flavonoids from stems and leaves of Calophylluminophyllum. Journal of Chinese Medicinal Materials. 2007;32:692- 694.

Raji P, Abila MG. Antony V. Samrot. A study on the antifeedant activity of Cassia fistula leaves Journal of Chemical and Pharmaceutical Research.2016;8(6):233-236.

RubelRanaMd. ,Nowrin Islam Amin, Abdullah An Naser, Nurul Islam. Screening of CalophylluminophyllumL. leaf extracts for cytotoxic, larvicidal, insect repellent and antimicrobial activities Journal of Pharmacognosy and Phytochemistry. 2017;6(3):612-616.

Abbott WS. A method of computing the effectiveness of an insecticide. J Econ Entomol.1925;18:265-267.

Finney DJ. Probit analysis. 3rd ed. Cambridge: Cambridge University Press; 1971.

Tauber OE, Yeager JF. On total hemolymph (blood) cell counts of insects I. Orthoptera, odonata, hemiptera and homoptera. Ann. Entomol. Soc. Am.1935;28:229- 240.

Jones JC. Current concepts concerning insect hemocytes. Am. Zool.1962;2:209-246.

Nittono Y. Studies on the blood cells in the silkworm, Bombyxmori L. Bull. Seric. Expt. Sta.1960;16: 171-266.

Koul O, WaliaiS, Dhaliwal GS. Essential oils as green pesticides: Potential and constraints. Biopesticides International. 2008;4(1):63–84.

Luximon-Ramma A, Bahorun T, Soobrattee MA, AruomaOI. Antioxidant activities of phenolic, proanthocyanidins, and flavonoid components in extracts of Cassia fistula, J. Agric. Food Chem. 2002; 50:5042-5047.

Samsudin. Biosynthesis and mode of actions of azadiracthinas an active ingredient of botanical insecticides, National Seminar IV on Botanicals Pesticides, Research Institute of Spices and Miscellaneous Industrial Plants (BALITRI), Sukabumi, Indonesia; 2011.

Saxena BP, Tikku K. Effect of plumbagin on haemocytes of Dysdercuskoenigii F. Proc. Indian Acad. Sci. (Anim. Sci.). 1990;99:119-124.

SharmaPR, Sharma OP, Saxena BP. Effects of neemgold on haemocytes of the tobacco armyworm, Spodopteralitura (Fabr.) (Lepidoptera: Noctuidae). Curr. Sci.2003;84:690-695.

Pandey JP, Upadhyay AK, Tiwari RK. Effect of some plant extracts on haemocyte count and moulting of Danaischrysippus larvae. J. Adv. Zool.2007;28:14-20.

George PJE, Ambrose DP. Impact of insecticides on the haemogram of Rhynocoriskumarii ambrose and livingstone (Hem.,Reduviidae). J. Applied Entomol.2004;128:600-604.