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The present investigation was undertaken to study the toxic effect of the insecticide Malathion (an Organo phosphate- pesticide) on biochemical parameters of the fresh water fish Labeo rohita (Hamilton). The fish were exposed to sub lethal concentration of the insecticide Malathion and the variations were observed on different parameters i.e. proteins, carbohydrates and ninhydrine positive substances (FAA) at different time intervals i.e. 24, 48, 72 and 96 hrs of Malathion exposure from different tissues of fish Labeo rohita i.e. gill, liver, intestine, muscle and brain. The results revealed that biochemical constituents i.e. proteins, carbohydrates and ninhydrine positive substances were decreased in all the tissues of L.rohita. The toxic nature of insecticide Malathion was found to be more after 48 hrs of exposure as highest % of decease was found in biochemical constituents of all the tissues.
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