EVALUATION OF ACUTE TOXICITY STUDIES OF DRAGON FRUIT (Hylocereus undatus) FLESH IN FINGERLINGS (Labeo rohita)
UTTAR PRADESH JOURNAL OF ZOOLOGY,
Recently, the fruits of Hylocereus undatus, known as dragon fruit, have received much attention from growers worldwide. However, there is little toxicological information regarding the safety of repeated exposure to these fruits. The present study evaluated the toxicity of H. undatus fruit flesh after acute administration in fish. In the acute toxicity study, Fingerling were not fed during the experimental period. Various concentrations (100, 250, 500, 1000 and 2000mg/L) of the test solutions were prepared from Hylocereus undatus stock solutions. A group of 10 laboratory acclimatized fingerling of a particular species having the same weight, size and age were introduced into each test concentration of Hylocereus undatus. After 24, 48, 72 and 96hrs monitor the mortality of fish. LC50 values were 48, 72 and 96hrs were more than 371.53mg/l, 3.46mg/l and 1.41mg/l observed respectively. The toxicity study which is essential for an adaptation of the traditional medicine was conducted to identify the tolerance limits of Hylocereus undatus fruit extract. According the obtained LD50 (446.68mg/L) values. These findings support the common practice of administration of Hylocereus undatus fruit as a medicinal decoction in herbal medicine.
- Hylocereus undatus
- acute toxicity study
- various concentrations
How to Cite
Kale OE, Awodele O, Akindele AJ. Subacute and subchronic oral toxicity assessments of Acridocarpus smeathmannii (DC.) Guill. & Perr. Root in Wistar rats, Toxicol. Rep. 2019; 161–175.
Sharif H, Mukhtar M, Mustapha Y, Baba G, Lawal A. Acute and subchronic toxicity profile of Euphorbia pulcherrima methanol extract on Wistar albino rats. Adv. Pharm. J. 2015;9-13.
Moreira SS, Tamashiro LK, Jorge BC, Balin PS, Heredia-Vieira SC, Almeida GL, Cardoso CAL, Kassuya CAL, Arena AC, Toxicological safety evaluation in acute and 28-day studies of aqueous extract from Serjania marginata Casar. (Sapindaceae) levels in rats. J. Ethnopharmacol; 2018.
Boelsterli UA, Mechanistic Toxicology. The Molecular Basis of How Chemicals Disrupt Biological Targets, 1st ed., Taylor and Francis Inc., London and New York. 2003;1–314.
Saad B, Azaizeh H, Abu-Hijleh G, Said S. Safety of traditional Arab herbal medicine. Evidence-based Complementary and Alternative Medicine. 2006;3:433–439.
Guengerich FP, Mechanisms of drug toxicity and relevance to pharmaceutical development. Drug Metabolism and Pharmacokinetics (DMPK), Advance Publication by J-STAGE, 2010;25-31.
Sprague JD. The ABC of Pollution Assays using Fish, Biological Methods for Assessment of Water Quality, A.S.T.M. STP. 1973;528: 6-30.
Finney DJ. Probit analysis. Cambridge University Press, New York, USA. 1971;337.
Saad B., H. Azaizeh, G. Abu-Hijleh, O. Said, Safety of traditional Arab herbal medicine, Evid.-Based compl. Altern. Med. 2006a;433–439.
Springfield EP, Eagles PKF, Scott G. Quality assessment of South African herbal medicines by means of HPLC fingerprinting. Journal of Ethnopharmacology. 2005;101:75–83.
Arome D, Chinedu E. The importance of toxicity testing. Journal of Pharmaceutical and BioScience. 2014;4:146–148.
Seth SD, Sharma B. Medicinal plants in India. Indian J Med Res. 2004;120:9-11.
Gupta M, Mazumder U, Kumar T, Gomathi P, Kumar R. Antioxidant and hepatoprotective effects of Bulhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats. Iran J. Pharmacol. Therapy. 2004;3:12-20.
OECD. Acute Oral Toxicity-up and down procedure. Guidelines for Testing of Chemicals. 2001;425:1-26.
Ribeiro LC. Investigação do efeito ictiotóxico do extrato etanolico da raiz de Spilanthes acmella (jambu) em zebrafish através da análise eletrofisiológica e comportamental. 61 f. Master’s Thesis, Programa de Pós-Graduação em Neurociências e Biologia Celular da Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, Brasil; 2013.
Little EE, Fairchild JF, Delonay AJ. Behavioral Methods for Assessing Impacts of Contaminants on Early Life Stage Fishes; American Fisheries Society: Bethesda, MD, USA. 1993;67–76.
Santos IVF, Duarte JL, Fernandes CP, Keita H. Amado JRR, Velázquez-Moyado JA, Navarrete A, Carvalho JCT. Use of zebrafish (Danio rerio) in experimental models for biological assay with natural products. Afr. J. Pharm. Pharmacol. 2016;10:883–891.
Everds NE, Snyder PW, Bailey KL, Bolon B, Creasy DM, Foley GL, Rosol TJ, Sellers T. Interpreting stress responses during routine toxicity studies: A review of the biology, impact, and assessment. Toxicol. Pathol. 2013; 41:560–614.
Abstract View: 254 times
PDF Download: 14 times