EVALUATION OF OXIDATIVE STRESS IN AZOXYMETHANE-INDUCED COLON CANCEROUS FISCHER RATS
UTTAR PRADESH JOURNAL OF ZOOLOGY,
Objective: To study the redox status of normal colon and aberrant crypts formed in azoxymethane induced colon cancerous fischer rats.
Methods: A total of 16 five-week-old male Fisher 344 rats (Rattus norvegicus), weighing approximately 90–100 grams were housed individually in plastic cages with wood-chip bedding. The animals were acclimatized for 1 week and fed with an American Institute of Nutrition (AIN-93G) diet ad libitum. Their protein oxidation, DNA damage, lipid peroxidation and antioxidants, glutathione (GSH), and antioxidative enzymes in serum were detected.
Results: The levels of protein oxidation and lipid peroxidation were significantly higher in the study group than in the control group (P<0.01). However, the mean serum level of MDA and conjugated diene was lower in the study group than in the control group (P<0.01). The activity of antioxidative enzymes was significantly decreased in the study group compared to control group (P<0.01).
Conclusion: Colorectal cancer is associated with oxidative stress, and assessment of oxidative stress and antioxidantsincorporation could be an important choice for the treatment and prevention of colorectal cancer.
- Colorectal cancerl azoxymethanel fischer ratsl oxidative stress
- lipid peroxidation
- protein oxidation
How to Cite
Gombart AF, Pierre A, Maggini S. A Review of micronutrients and the immune system-working in harmony to reduce the risk of infection. Nutrients. 2020;12 (1): 236.
Ohashi W, Fukada T. Contribution of Zinc and zinc transporters in the pathogenesis of inflammatory bowel diseases. J imm res. 2019; 83(9):68-78.
Maares M, Haase H. A guide to human zinc absorption: general overview and recent advances of in vitro intestinal models. Nutrients. 2020;12(3):762.
Michalczyk K, Cymbaluk-Płoska A. The role of zinc and copper in gynecological malignancies. Nutrients. 2020;12(12): 3732.
Mehta S, Shelling A, Muthukaruppan A, Lasham A, Blenkiron C, Laking G, Print C. Predictive and prognostic molecular markers for cancer medicine. Ther Adv Med Oncol. 2010;2(2):125–148.
Dhawan DK, Chadha VD. Zinc: A promising agent in dietary chemoprevention of cancer. The Indian Journal of Medical Research. 2010;132(6):676–682.
Claudia A, Lucia B, Ivano B, Antonio R. Zinc through the three domains of life. J Prot Res. 2006;5:3173-8.
Sharma Ankita, Sharma Dixit, Verma Shailender K. In silico study of iron, zinc and copper binding proteins of Pseudomonas syringae pv. lapsa: Emphasis on Secreted Metalloproteins. Fron Microbiol. 2008;9:1838.
Prasad AS. Zinc in human health: Effect of zinc on immune cells. Mol. Med. Camb. Mass. 2008;14:353–357.
Prasad AS. Impact of the discovery of human zinc deficiency on health. J. Trace Elem. Med. Biol. 2014;28:357–363.
Fukada T, Yamasaki S, Nishida K, Murakami M, Hirano T. Zinc homeostasis and signaling in health and diseases: Zinc signaling. J Biol Inorg Chem. 2011;16:1123–1134.
Fukada T. Zinc biology and zinc signaling. Biomed. Res. Trace Elem. 2015; 26:1–6.
Dani V, Ajay Goel, Vaiphei K, Dhawan, DK. Chemopreventive potential of zinc in experimentally induced colon carcinogenesis. Toxicol Lett. 2007;171:10–18.
Tanaka T. Colorectal carcinogenesis: Review of human and experimental animal studies. J Carcinog. 2009;8:5.
Satya Prasad PPV, Sreedhar B, Vijaya Lakshmi B. Dietary phytate lowersiron status, suppresses aberrant crypt foci and inhibits progression of adenoma in azoxymethane-induced colon cancer, Int J Pharm Biol Sci. 2018;2(8):43-55.
Kavitha V, Naveena Lavanya Latha J, Vijaya Lakshmi B, Zinc dyshomeostasis in azoxymethane-induced colonic precancerous and cancerous lesions in Fischer rats, Metallomics. 2021;13(2):1-10.
Bird RP, Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: Preliminary findings, Cancer Let. 1987;37(2):147-51.
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882-888.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70:158-169.
Aebi H. Catalase in vitro. Meth Enzymol. 1984;105:121-126.
Sun Y, Oberley L W, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1998;34:497-500.
Reznick AZ, Packer L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Meth Enzymol. 1994;233:357-63.
Paul H, Russell H. Fluorimetric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem. 1976; 74:214-26.
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469-74.
Liu CM, Liang D, Jin J, Li DJ, Zhang YC, Gao ZY, He YT. Research progress on the relationship between zinc deficiency, related microRNAs, and esophageal carcinoma. Thor Can. 2017;8(6):549–557.
Pretlow TP, Brasitus TA, Fulton NC, et al. K-ras mutations in putative preneoplastic lesions in human colon. J Natl Cancer Inst. 1993; 85:2004-7.
Yamada Y, Mori H. Pre-cancerous lesions for colorectal cancers in rodents: A new concept, Carcinogen. 2003;24 (6):1015–1019
Reddy B, Rao C, Seibert K. Evaluation of cyclooxygenase-2 inhibitor for potential chemopreventive properties in colon carcinogenesis. Cancer Res. 2006;56:4566–4569.
Justin GW, Matthew SA. Joseph JC. Superoxide Dismutases in Pancreatic Cancer Antioxidants. 2017;6(3):66.
Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: Properties, sources, targets, and their implication in various diseases. Ind J Clin Biochem. 2019;30(1):11–26.
Christudoss P, Selvakumar R, Jude Joseph F, George M. Zinc levels in paired normal and malignant human stomach and colon tissue Biomed Res. 2010;21(4):445-450.
Zhou JF, Xu GB, Fang WJ. Relationship between acute organophosphorus pesticide poisoning and damage induced by free radicals. Biomed Environ Sci. 2002; 15:177-186.
Ozdemirler Erata G, KanbaÄŸli O, Durlanik O, et al. Induced oxidative stress and decreased expression of inducible heat shock protein 70 (ihsp 70) in patients with colorectal adenocarcinomas. Jpn J Clin Oncol. 2005; 35:74-78.
Skrzydlewska E, Sulkowski S, Koda M B, et al. Lipid peroxidation and antioxidant status in colorectal cancer. World J Gastroenterol. 2005;11(3):403-406.
Rainis T, Maor I, Lanir A, et al. Enhanced oxidative stress and leucocyte activation in neoplastic tissues of the colon. Dig Dis Sci. 2007;52:526-530.
Devasena T, Venugopal Menon P. Fenugreek seeds modulate 1, 2-dimethylhydrazine-induced hepatic oxidative stress during colon carcinogenesis. Ital J Biochem. 2007; 56:28-34.
Muse KE, Oberley TD, Sempf JM, et al. Immunolocalization of antioxidant enzymes in adult hamster kidney. Histochem J. 1994;26: 734-753.
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