A PRELIMINARY COMPARATIVE STUDY OF BACTERIAL COLONIES ISOLATED FROM THE ORAL CAVITY OF A BETEL QUID CHEWER AND A NON-CHEWER BEFORE AND AFTER TREATMENT WITH NATURAL PRODUCTS

RIMPA LAHKAR; BANDITA BARUWA; PRITAM DEY SARKAR

A PRELIMINARY COMPARATIVE STUDY OF BACTERIAL COLONIES ISOLATED FROM THE ORAL CAVITY OF A BETEL QUID CHEWER AND A NON-CHEWER BEFORE AND AFTER TREATMENT WITH NATURAL PRODUCTS

RIMPA LAHKAR

BANDITA BARUWA

PRITAM DEY SARKAR

UTTAR PRADESH JOURNAL OF ZOOLOGY, Page 57-64
Published: 6 December 2021

Abstract

The present study aims at a preliminary investigation of antibacterial properties of Neem, Charcoal and husk of betel nut in case of betel quid chewers and non-chewers and characterization of oral bacteria before and after treatment with raw neem, charcoal and husk of betel nut. Buccal swab samples were collected from oral cavities of chewer and non-chewer using sterile cotton swabs and streaked on sterile nutrient agar plates. The plates were kept for overnight incubation and bacterial colonies were observed. Colony morphology was studied followed by Gram staining. More bacterial colonies were obtained in case of chewer as compared to that of non- chewer. Gram-positive cocci (GPC), few gram-positive bacilli (GPB) and gram-negative cocci (GNC) were observed. After treatment with Neem significant reduction in GPC was observed. Neem showed the highest reduction in bacterial colonies followed by Charcoal and husk of betel nut.

Keywords:

Antibacterial
betel nut
areca
neem
charcoal

PDF Requires Subscription or Fee (USD 30)

How to Cite

LAHKAR, R., BARUWA, B., & SARKAR, P. D. (2021). A PRELIMINARY COMPARATIVE STUDY OF BACTERIAL COLONIES ISOLATED FROM THE ORAL CAVITY OF A BETEL QUID CHEWER AND A NON-CHEWER BEFORE AND AFTER TREATMENT WITH NATURAL PRODUCTS. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(24), 57-64. Retrieved from https://mbimph.com/index.php/UPJOZ/article/view/2655

References

Anand R, Dhingra C, Prasad S, Menon I. Betel nut chewing and its deleterious effects on oral cavity. Journal of cancer research and therapeutics. 2014;10(3):499–505. Available:https://doi.org/10.4103/0973-1482.137958

Saini S, Saini SR, Katiyar R, Bhalerao DS, Munde A. The use of tobacco and betel leaf and its effect on the normal microbial flora of oral cavity.Pravara Medical Review. 2009;4 (4):17-19.

Benegal V, Rajkumar RP, Muralidharan K. Does areca nut use lead to dependence? Drug and alcohol dependence. 2008;97(1-2):114–121. Available:https://doi.org/10.1016/j.drugalcdep.2008.03.016

Chandak R, Chandak MG, Rawlani S. Current Concepts about Areca Nut Chewing. ACM Journal of Computer Documentation. 2013;3:78-81.

Hazarika P, Dutta D. Traditional knowledge for using plant resources as tooth brushing stick (datun) by the indigenous communities of Assam, India. International Journal of Herbal Medicine. 2018;6(6):22-34.

Lakshmi, Dr, Kumar Subramanian, Aravind. Antibacterial evaluation of Azadirachta Indica ethanolic leaf extract against selected acidogenic oral bacteria causing dental plaque in fixed orthodontic appliance patients - An Invitro study. International Journal of Dental Research & Development (IJDRD). 2014;4:7-12.

Banerjee S, Kim LM, Shariff HK, Yusoff FM. Antibacterial Activity of Neem (Azadirachtaindica) Leaves on Vibrio spp. Isolated from Cultured Shrimp. Asian Journal of Animal and Veterinary Advances. 2013; 8(2): 355-361.

Ahuja SC, Ahuja U. Betel leaf and Betel Nut in India: History and Uses, Asian Agri-History. 2011;15(1):13-35.

Maragathavalli S, Brindha S, Kaviyarasi NS, Annadurai B, Gangwar SK. Antimicrobial activity in leaf extract of neem. International Journal of Science and Nature. 2012;3(1):110-113.

Jose M, Cyriac MB, Vidya P, Varghese I, Shantaram M. Anti-microbial properties of Areca catechu (areca nut) husk extracts against common oral pathogens. International Journal of Research in Ayurveda and Pharmacy. 2012;3(1): 81-84.

Yang FC, Wu KH, Lin WP, Hu MK, Sheu TS, Horng DN. Preparation and Anti-bacterial effect of Bamboo charcoal-silver on Staphylococcus aureus and Pseudomonas aeruginosa, Journal of the Chinese chemical society. 2009;56(2): 327-330.

Arathi G, Venkateshbabu N, Deepthi M, Jayaleelashri J, Saranya V, Kandaswamy D. In-vitro antimicrobial efficacy of aqueous extract of Areca nut against Enterrococcus faecalis. Indian Journal of Research in Pharmacy and Biotechnology. 2015;3(2):147-150.

Dubey RC, Maheshwari DK. Practical Microbiology. New Delhi: S. Chand; 2002.

Yerima MB. Jodi, SM. Oinbo K, Maishanu HM. Farouq AA. Junaidu AU. Al-Mustapha MN, Shinkafi AL. Effect of Neem extracts (Azadirachtaindia) on Bacteria isolated from adult mouth, Nigerian Journal of Basic and Applied Sciences. 2012;20 (1): 64-67.

Choi MS, Ahn KS. Anti-bacterial effect of bamboo charcoal on Streptococcus mutans. Journal of Korean society of Dental Hygiene. 2014;14(1): 95-100.

334 times
12 times