EFFECT OF MICRONUTRIENTS SUPPLEMENTED MULBERRY LEAVES ON THE LARVAL BIOCHEMICAL CHARACTERISTICS OF MULBERRY SILKWORM, Bombyx mori LINNAEUS 1758 (LEPIDOPTERA: BOMBYCIDAE)
UTTAR PRADESH JOURNAL OF ZOOLOGY,
Nutritive value of mulberry leaf is a key factor besides environment and technology adoption for better growth and development of the silkworm larvae. The present study deals with the effect of micronutrient supplemented mulberry leaves on the biochemical characteristics of PM´CSR2 hybrid variety of mulberry silkworm, Bombyx mori fifth instar larvae. The field experiment was laid out in a randomized block design with twelve treatments including a control (T0 to T11), and the treatments were replicated thrice. Each treatment was supplemented with the desired quantity of the respective micronutrient in single or in combination. Twenty early Bombyx mori fifth instar larvae in plastic trays were used for each replicate of the respective treatment, and were fed with the 5-6 fully grown mature mulberry leaves (T0 to T11) twice a day (morning and evening). The amount of carbohydrate and protein present in haemolymph, silk gland, fat body and muscle, and the amount of lipid, trehalose and glycogen in haemolymph and fat body of the fifth instar larvae of control and treated group were estimated. Maximum carbohydrate content in the haemolymph, silk gland, fat body and muscle of the fifth instar Bombyx mori was observed in T9 (195.0 ±7.07mg/mL), both T8 and T9 (190 ±13.13mg/g), T8 (245.0 ±49.49mg/g) and T9 (152.5 ±16.6mg/g) respectively, and for the maximum protein content, the respective values were in T9 (158.0 ±31.11mg/mL), T9 (172.0 ±11.31mg/g), T8 (166.0 ±76.36mg/g) and T9 (195.0 ± 86.26mg/g). The maximum lipid, trehalose and glycogen in haemolymph was observed in T8 (5.96 ±1.40mg/mL), T3 (7.66 ±1.25mg/mL) and T5 (1.30 ±2.51mg/mg/mL) respectively, and for maximum content in fat body, the respective values were observed in T6 (16.87 ±1.20mg/mL), T9 (7.91 ±0.24mg/mL) and T8 (11.87 ±0.10mg/mL) Overall results of the present study emphasized that supplemented micronutrients in T9 (CuSO4 15Kg/ha + ZnSO4 15Kg/ha + FeSO4 30Kg/ha) followed by T8 (CuSO4 10Kg/ha + ZnSO4 10Kg/ha + FeSO4 20Kg/ha) positively influenced the biochemical characteristics of Bombyx mori fifth instar larvae.
- Bombyx mori larva
- silk gland
- fat body
How to Cite
Chandrakala, Sadatulla F. Effect of soil application of zinc, iron and manganese on V1 mulberry and total performance of cross breed PM×CSR2 (Bombyx mori L.). Journal of Entomology and Zoology Studies. 2020; 8(2):712-716.
Krishnaswami S, Kumararaj S, Vijayaraghavan K, Kasiviswanathan K. Silkworm feeding trials for evaluating the quality of mulberry leaves as influenced by variety, spacing and nitrogen fertilization. Indian Journal of Sericulture. 1971;10:79-89.
Krishnaswami S, Narasimhanna MN, Suryanarayan SK, Kumararaj S. Manual of sericulture. Silkworm rearing. Volume 2. FAO, Rome. 1973,6871.
Choudhury PC, Shuka P, Chosh B, Sengupta K. Effect of spacing, crown height and method of pruning on mulberry leaf yield, quality of cocoon yield. Indian Journal of Sericulture. 1991;30(1):46-53.
Etebari K, Kaliwal BB, Matindoost L. Different aspects of mulberry leaves supplementation with various nutritional compounds in sericulture. International Journal of Industrial Entomology. 2004;9(1):15-28.
Slansky FJ, Scriber JM. Food consumption and utilization. In: Comprehensive Insect Physiology, Biochemistry and Pharmacology. Kerkut GA, Gilbert LI (eds.), Volume 4, Pergamon Press, New York. 1985,88-163.
Krishnaswami S. New technology of silkworm rearing. Bulletin of the Central Sericultural Research and Training Institute, Mysore, India. 1978,1-10.
Lowry OH, Rosebrough N, Farr A, Randall R. Protein measurement with folin phenol reagent. Journal of Biological Chemistry. 1951;193:265-275.
Floch J, Lee M, Stanley GHS. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry. 1957;226(1):497-509.
Sadasivam S, Manickam A. Biochemical methods. New Age International Limited Publishers, 3rd Edition, New Delhi. 2008, 270.
Roe JH. The determination of sugar in blood and spinal fluid with anthrone reagent. Journal of Biological Chemistry. 1955;212(1):335-343.
Seifter S, Dayton S, Novic B, Muntwyler E. The estimation of glycogen with the anthrone reagent. Archives of Biochemistry. 1950;25(1):191-200.
Ito T, Horie Y. Carbohydrate metabolism of the midgut of the silkworm, Bombyx mori L. Archives of Biochemistry and Biophysics. 1959;80:174-176.
Wyatt GR. The biochemistry of insect haemolymph. Annual Review of Entomology. 1961;6:75-102.
Wyatt GR. The biochemistry of sugars and polysaccharides in insects. Advances in Insect Physiology. 1967;4:287-360.
Kanafi RR, Ebadi R, Mirhosseini SZ, Seidavi AR, Zolfaghari M, Etebari K. A review on nutritive effect of mulberry leaves enrichment with vitamins on economic traits and biological parameters of silkworm Bombyx mori L. Invertebrate Survival Journal. 2007;4(2):86-91.
Periasamy K, Narayanaprakash R, Radhakrishnan S. Food utilization in exotic and indigenous races of Bombyx mori L. (Lepidoptera: Bombycidae). Sericologia. 1984;24:43-50.
Anandakumar MD, Michael AS. Effect of nutritive additive of mulberry and its impact on nutritional components of silkworm Bombyx mori L. International Journal of Advanced Biotechnology and Research. 2012;3(1):523-529.
Bahar MH, Parvez MA, Rahman S, Islam R. Performance of polyvoltine silkworm Bombyx mori L. on different mulberry plant varieties. Entomological Research. 2011;41: 46-52.
Zhang PB, Aso YK, Jikuya H, Kusakabe T, Lee JM, Kawaguchi Y, Yamamoto K, Banno Y, Fujii H. Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. Journal of Proteome Research. 2007;6:2295-2303.
Sivaprasad S, Mohan MP. Neuromuscular systems in the fifth instar larva of the silkworm, Bombyx mori (Lepidoptera: Bombycidae): I- Cephalothoracic musculature and its innervation. Journal of Applied and Natural Science. 2009;1:201-209.
Sivaprasad S, Mohan MP. Neuromuscular systems in the fifth instar larva of the silkworm, Bombyx mori. (Lepidoptera: Bombycidae): II- Abdominal musculature and its innervation. Journal of Applied and Natural Science. 2009;1:210-226.
Creighton TE. Protein folding. Biochemical Journal. 1990;270:1-16.
Zhou L, Li H, Hao F, Li N, Liu X, Wang G, Wang Y, Tang H. Developmental changes for the hemolymph metabolome of silkworm (Bombyx mori L.). Journal of Proteome Research. 2015;14(5):2331-2347.
Shen W, Han W, Li Y, Meng Z, Cai L, Li L. Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics. Analytica Chimica Acta. 2016; 942:1-11.
Dong HL, Zhang SX, Tao H, Chen ZH, Li X, Qiu JF, Cui WZ, Sima YH, Cui WZ, Xu SQ. Metabolomics differences between silkworms (Bombyx mori) reared on fresh mulberry (Morus) leaves or artificial diets. Scientific Reports. 2017;7(1):10972.
Lix H, Wu XF, Liu JM, Li GL, Miao YG. Proteomic analysis of the silkworm (Bombyx mori L) haemolymph during developmental stage. Journal of Proteome Research. 2006;5:2809-2814.
Pawar VM, Ramakrishna N. Biochemical changes in larval haemolymph of Spodoptera litura Fabricius due to nuclear polyhedrosis virus infection. Indian Journal of Experimental Biology. 1977;15:755-758.
Rajitha K, Savithri G. Day to day analysis of amylase and trehalase activity in the haemolymph of silkworm Bombyx mori L. infected with fungal pathogen Beauveria bassiana (Bals.) Vuill. International Journal of Life Sciences Biotechnology and Pharma Research. 2014;3(1):225-230.
Padmaja PR. Some aspects of physiology of the eri silkworm, Philosamia ricini. Ph.D. Thesis, Karnatak University, Dharwad, India. 1991.
Saha BN, Khan AR. The growth and development of the silkworm, Bombyx mori L. on feed supplemented with nicotinic acid. Bangladesh Journal of Life Sciences. 1996;1:103-109.
Hou Y, Zhao P, Liu HL, Zou Y, Guan J, Xia QY. Proteomics analysis of fat body from silkworm (Bombyx mori). Shengwu Gongcheng Xuebao. 2007;23(5): 867-872.
Bhattacharya A, Kaliwal BB. The biochemical effects of potassium chloride on the silkworm Bombyx mori L. Insect Science. 2005;12:95-100.
Bhattacharya A, Kaliwal BB. Synergetic effects of potassium and magnesium chloride on the biochemical contents of the silkworm Bombyx mori. L. Caspian Journal of Environmental Sciences. 2005;3(1):15-21.
Bhattacharya A, Kaliwal BB. Fortification of mulberry leaves with mineral magnesium chloride (MgCl2) on biochemical contents of the silkworm, Bombyx mori L. The Philippine Agricultural Scientist. 2005;38(3):337- 340.
Downer RGH, Matthews JR. Patterns of lipid distribution and utilization in insects. American Zoologist. 1976;16:733-745.
Satake S, Kawabe Y, Mizoguchi A. Carbohydrate metabolism during starvation in the silkworm Bombyx mori L. Archives of Insect Biochemistry and Physiology. 2000;44:90-98.
Simex V, Kodrik D. Changes in the tissue glycogen and free carbohydrates of haemolymph during the last larval instar and metamorphosis of silkworm Bombyx mori L. Acta Entomologica Bohemoslovaca. 1986;83(2):92-100.
Nirwani RB, Kaliwal BB. Effect of ferrous and magnesium sulphate supplementation on some commercial characters of B. mori L. Bulletin of Sericultural Research. 1995;6:21-27.
Pant R, Morris ID. Changes in active phosphorylase activity and glycogen content during larval and pupal development of Philosamia ricini. Journal of Biochemistry (Tokyo). 1969;66:29-31.
Abstract View: 404 times
PDF Download: 34 times