THE EFFECT OF BmNPV INFECTION AND SUBSEQUENT ORAL TREATMENT OF ETHANOLIC PLANT EXTRACTS ON SDS-PAGE PROTEIN PROFILE OF SILKWORM PM B. mori L.
J. A. CHAVAN
*
Department of Zoology, Rajaram College, Kolhapur (MS), 416 008, India.
L. P. BHOPALE
Department of Zoology, Rajaram College, Kolhapur (MS), 416 008, India.
S. S. TAWARE
Department of Zoology, Rajaram College, Kolhapur (MS), 416 008, India.
*Author to whom correspondence should be addressed.
Abstract
In the present study effect of BmNPV infection and subsequent oral treatment of ethanolic plant extracts on SDS-PAGE protein profile of midgut, silkgland, fatbody and haemolymph on 3rd and 5th day of fifth instar larvae of silkworm PM B. mori L. were studied. The electrophoretic analysis of proteins in midgut, silkgland, fatbody and haemolymph revealed the separation of maximum protein bands among which some of them represents the storage proteins. The BmNPV inoculation causes decrease in number of protein bands and staining intensity was also low as compared to the control. The treatment of plant extracts shows the decreased protein bands and staining intensity low but in some group shows the similar protein bands when compared with the control group sample.
Keywords: Bombyx mori, BmNPV, Protein profile, SDS-PAGE